ELISA/assay

KRIBIOLISA™ Protein A ELISA

KBBP11

1 x 96 wells

320 USD

KRIBIOLISA™ Protein A ELISA

ELISA

1 x 96 wells

320 USD

This kit is used for determination of natural and recombinant constructs of Protein A ligand contamination, in various antibody products that are purified through Protein A chromatographic techniques

Human

A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals. These antibodies are generally purified through protein A column chromatography. This method of purification has the potential with contamination of protein A, which might get leached out of the column and co-eluted with purified antibody. This may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody. The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as well as screening product prior to its use.

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

2-8 Deg C

0 pg/ml - 4000 pg/ml

0.5 ng/ml

This assay is based on the principle of Sandwich ELISA. Samples containing Protein A are reacted with antiProtein A antibody coated on the microtitre plates. The wells are washed to remove any unbound reactants as per the wash procedure. Anti-Protein A HRP antibody is added to the wells and incubated. The wells are washed to remove any unbound reactants as per the wash procedure. simultaneously in the microtiter wells already coated with affinity purified capture anti-Protein A antibody. The substrate 3,3’,5,5’ Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of Protein A present.

protein A

RUO

2-8 degrees C