ELISA/assay

KRIBIOLISA™ Bovine Serum Albumin ELISA

KBBP10

1 x 96 wells

350 USD

KRIBIOLISA™ Bovine Serum Albumin ELISA

ELISA

1 x 96 wells

350 USD

Manufacturing of Biopharmaceutical products using cells or tissue culture can result in residual impurities of the desired product by components used in the culture media. Most commercial formulations contain significant amounts of albumin and transferrin either of bovine or human origin, and insulin from various species.This kit is used for determination of BSA contamination in various antibody products that are purified through immobilized BSA chromatographic techniques

Human

The KRIBIOLISA™ ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

2-8 Deg C

2.5 ng/ml - 80 ng/ml

2 ng/ml

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Bovine Serum Albumin present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Bovine Serum Albumin in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Serum Albumin

RUO

2-8 degrees C