ELISA/assay

KRIBIOLISA™ Human IgG ELISA

KBBP08

1 x 96 wells

230 USD

KRIBIOLISA™ Human IgG ELISA

ELISA

1 x 96 wells

230 USD

This generic kit is intended in determining the presence of Total human Immunoglobulin G contamination in variety of sample types A highly sensitive and specific Enzyme linked immunosorbent assay can be used for the quantitation of low levels of human IgG

Human

Human IgG is a monomeric immunoglobulin, and the most abundant one constituting approximately 75% of the serum. Presence of such trace contaminants in the products may lead to potential health hazards, especially when the product is intended for therapeutic use.

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

2-8 Deg C

0 ng/ml - 50ng/ml

0.17ng/ml

This assay is based on the Sandwich ELISA procedure. Samples containing hIgG are reacted with anti hIgG-HRP antibody simultaneously in the microtiter wells already coated with affinity purified capture IgG antibody. This immunological reaction results in formation of a sandwich complex of solid phase antibody-hIgG-enzyme labeled antibody. The wells are washed to remove any unbound reactants as per the wash procedure (see Assay procedure section mentioned below). The substrate 3, 3’, 5, 5’ Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of hIgG present.

IgG

RUO

2-8 degrees C