ELISA/assay

KRIBIOLISA™ Human Serum Albumin ELISA

KBBP07

1 x 96 wells

230 USD

KRIBIOLISA™ Human Serum Albumin ELISA

ELISA

1 x 96 wells

230 USD

Immunoassay for the measurement of Human Serum Albumin (HSA) in cell culture supernantant and biological preparations. A variety of products, manufactured using the biotechnology route such as cell or tissue culture may result in residual contamination of the end-product. Trace contamination of HSA in pharmaceutical and other biological products is one such area. Such trace contaminants may lead to potential health hazards in humans, especially when the product is intended for therapeutic use. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year This assay is based on the Sandwich ELISA method. Samples containing Human Albumin are reacted with microtiter wells pre-coated with affinity purified capture HSA antibody followed by washing and then detected using detection antibody and HRP Conjugated antibody. This immunological reaction results in formation of an immuno complex of solid phase. The wells are washed to remove any unbound reactants. The substrate 3, 3’, 5, 5’ Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of HSA present.

A variety of products, manufactured using the biotechnology route such as cell or tissue culture may result in residual contamination of the end-product. Trace contamination of HSA in pharmaceutical and other biological products is one such area. Such trace contaminants may lead to potential health hazards in humans, especially when the product is intended for therapeutic use.

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

2-8 Deg C

0.5 ng/ml - 100 ng/ml

1 ng/ml

This assay is based on the Sandwich ELISA method. Samples containing Human Albumin are reacted with microtiter wells pre-coated with affinity purified capture HSA antibody followed by washing and then detected using detection antibody and HRP Conjugated antibody. This immunological reaction results in formation of an immuno complex of solid phase. The wells are washed to remove any unbound reactants. The substrate 3, 3’, 5, 5’ Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of HSA present.

Serum Albumin

RUO

2-8 degrees C