ELISA/assay

KRIBIOLISA™ SP 2/0 HCP ELISA

KBBP05

1 x 96 wells

500 USD

KRIBIOLISA™ SP 2/0 HCP ELISA

ELISA

1 x 96 wells

500 USD

This generic kit is intended in determining the presence of SP2/0 Host Cell Proteins contamination in various products that are manufactured through recombinant expression in SP2/0 cells The kit has been validated successfully for testing of in process and final product HCPs in variety of products regardless of growth and purification process.

Human

Recombinant expression by the hybridoma cell line SP2/0 is a relatively simple and cost effective method for production of monoclonal antibodies intended for use as therapeutic agents in humans.

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

2-8 Deg C

0 ng/ml - 300 ng/ml

1 ng/ml

This assay is based on the Sandwich ELISA procedure. Microwells are coated with anti- SP2/0 antibody. Addition of Anti- SP2/0:HRP Conjugate and standards or samples containing SP2/0 HCPs will form a immune complex with the coated anti- SP2/0 antibody in microwell. A washing cycle will remove free Anti- SP2/0:HRP Conjugate from the reaction well. Addition of TMB Substrate will produce blue color in microwells conataining immune complex. Addition of stop solution will terminate the enzymatic reaction and blue color will be converted to yellow color which is directly proportional to the concentration of SP2/0 HCPs present and absorbance value is measured at 450nm.

SP 2/0 HCP

RUO

2-8 degrees C