KRIBIOLISA™ CHO HCP ELISA | Krishgen Biosystems KBBP03 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

KRIBIOLISA™ CHO HCP ELISA

catalog :

KBBP03

quantity :

1 x 96 wells

price :

360 USD

more info or order :

Krishgen Biosystems product webpage

product information

Catalog Number :

KBBP03

Product Name :

KRIBIOLISA™ CHO HCP ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

360 USD

Product Description :

This generic kit is intended in determining the presence of Chinese Hamster Ovary Host Cell Proteins contamination in various products that are manufactured through recombinant expression in CHO cells The kit has been validated successfully for testing of in process and final product HCPs in variety of products regardless of growth and purification process.

SpeciesSummary :

Human

Background :

A variety of proteins which are used as therapeutic agents in humans and animals are produced through recombinant expression in Chinese Hamster Ovary (CHO) cells. The manufacturing and purification process of these products tends to leave the potential for contamination by Host Cell Proteins (HCPs) from CHO cells, which may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the therapeutic agent. The simple, objective and semi-quantitative ELISA is a highly sensitive method that aids in purification process development, process control, quality control and product release testing optimally.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

6.25 ng/ml - 400 ng/ml

Sensitivity :

5 ng/ml

Assay Principle :

This assay is based on the Sandwich ELISA procedure. Samples containing CHO HCPs are reacted with already coated affinity purified capture anti-CHO HCP antibody. Following an incubation period unbound components are removed by a washing step. Then the anti-CHO HCP: HRP is added. This immunological reaction results in formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The wells are again washed to remove any unbound reactants. The TMB substrate is then added to the well. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of CHO HCPs present.

Biomarker :

CHO HCP

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage