GENLISA™ Rat TNF Alpha ELISA | Krishgen Biosystems KB3145 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Rat TNF Alpha ELISA

catalog :

KB3145

quantity :

1 x 96 wells

price :

270 USD

more info or order :

Krishgen Biosystems product webpage

citations: 20

Reference
Rana A, Singh S, Deshmukh R, Kumar A. Pharmacological potential of tocopherol and doxycycline against traumatic brain injury-induced cognitive/motor impairment in rats. Brain Inj. 2020;34:1039-1050 pubmed publisher
Asad M, Wajid S, Katare D, Mani R, Jain S. Differential Expression of TOM34, AL1A1, PADI2 and KLRBA in NNK Induced Lung Cancer in Wistar Rats and their Implications. Curr Cancer Drug Targets. 2019;19:919-929 pubmed publisher
Lakshminarayanashastry Viswanatha G, Venkatanarasappa Venkataranganna M, Lingeswara Prasad N. Methanolic leaf extract of Punica granatum attenuates ischemia-reperfusion brain injury in Wistar rats: Potential antioxidant and anti-inflammatory mechanisms. Iran J Basic Med Sci. 2019;22:187-196 pubmed publisher
Rahman S, Panda B, Parvez S, Kaundal M, Hussain S, Akhtar M, et al. Neuroprotective role of astaxanthin in hippocampal insulin resistance induced by Aβ peptides in animal model of Alzheimer's disease. Biomed Pharmacother. 2019;110:47-58 pubmed publisher
Danduga R, Reddy D, Seshadri S, Has K, Kumar K. Effect of combination therapy with pramipexole and n-acetylcysteine on global cerebral ischemic reperfusion injury in rats. Iran J Basic Med Sci. 2018;21:569-576 pubmed publisher
Kaundal M, Zameer S, Najmi A, Parvez S, Akhtar M. Betulinic acid, a natural PDE inhibitor restores hippocampal cAMP/cGMP and BDNF, improve cerebral blood flow and recover memory deficits in permanent BCCAO induced vascular dementia in rats. Eur J Pharmacol. 2018;: pubmed publisher
Ahmad S, Panda B, Fahim M, Dhyani N, Dubey K. Ameliorative Effect of Beraprost Sodium on Celecoxib Induced Cardiotoxicity in Rats. Iran J Pharm Res. 2018;17:155-163 pubmed
Kaur N, Jamwal S, Deshmukh R, Gauttam V, Kumar P. Beneficial effect of rice bran extract against 3-nitropropionic acid induced experimental Huntington's disease in rats. Toxicol Rep. 2015;2:1222-1232 pubmed publisher
Thakare V, Aswar M, Kulkarni Y, Patil R, Patel B. Silymarin ameliorates experimentally induced depressive like behavior in rats: Involvement of hippocampal BDNF signaling, inflammatory cytokines and oxidative stress response. Physiol Behav. 2017;179:401-410 pubmed publisher
Jamwal S, Singh S, Gill J, Kumar P. L-theanine prevent quinolinic acid induced motor deficit and striatal neurotoxicity: Reduction in oxido-nitrosative stress and restoration of striatal neurotransmitters level. Eur J Pharmacol. 2017;811:171-179 pubmed publisher
Kumar M, Kaur D, Bansal N. Caffeic Acid Phenethyl Ester (CAPE) Prevents Development of STZ-ICV Induced dementia in Rats. Pharmacogn Mag. 2017;13:S10-S15 pubmed publisher
Gill J, Jamwal S, Kumar P, Deshmukh R. Sertraline and venlafaxine improves motor performance and neurobehavioral deficit in quinolinic acid induced Huntington's like symptoms in rats: Possible neurotransmitters modulation. Pharmacol Rep. 2017;69:306-313 pubmed publisher
Akyol A, Şimşek M, Ilhan R, Can B, Baspinar M, Akyol H, et al. Efficacies of vitamin D and omega-3 polyunsaturated fatty acids on experimental endometriosis. Taiwan J Obstet Gynecol. 2016;55:835-839 pubmed publisher
Shukla G, Bhatia R, Sharma A. Prebiotic inulin supplementation modulates the immune response and restores gut morphology in Giardia duodenalis-infected malnourished mice. Parasitol Res. 2016;115:4189-4198 pubmed
Jamwal S, Kumar P. Spermidine ameliorates 3-nitropropionic acid (3-NP)-induced striatal toxicity: Possible role of oxidative stress, neuroinflammation, and neurotransmitters. Physiol Behav. 2016;155:180-7 pubmed publisher
Patel N, Bhutani K. Suppressive effects of Mimosa pudica (L.) constituents on the production of LPS-induced pro-inflammatory mediators. EXCLI J. 2014;13:1011-21 pubmed
Kothavade P, Bulani V, Nagmoti D, Deshpande P, Gawali N, Juvekar A. Therapeutic Effect of Saponin Rich Fraction of Achyranthes aspera Linn. on Adjuvant-Induced Arthritis in Sprague-Dawley Rats. Autoimmune Dis. 2015;2015:943645 pubmed publisher
Patel N, Pulipaka S, Dubey S, Bhutani K. Pro-inflammatory cytokines and nitric oxide inhibitory constituents from Cassia occidentalis roots. Nat Prod Commun. 2014;9:661-4 pubmed
Patel N, Bhutani K. Pinostrobin and Cajanus lactone isolated from Cajanus cajan (L.) leaves inhibits TNF-α and IL-1β production: in vitro and in vivo experimentation. Phytomedicine. 2014;21:946-53 pubmed publisher
Rafiq M, Viswanatha G, Suryakanth D, Azeemuddin M, Jagadeesh M, Dhanush K, et al. Poly-Ingredient Formulation Bresol(®) Ameliorates Experimental Chronic Obstructive Pulmonary Disease (COPD) in Rats. Sci Pharm. 2013;81:833-42 pubmed publisher

product information

Catalog Number :

KB3145

Product Name :

GENLISA™ Rat TNF Alpha ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

270 USD

Product Description :

This ELISA is intended for the quantitative detection of TNF-Alpha in rat serum, cell cuture supernatant and plasma samples. ELISA Features include:- Ready to use protocol, Standardisation and High Reproducibility, Lot to Lot Consistency, Accuracy and Precision. Validated against seven points for a Gold Ring Standard Quality ELISA - the benchmark sign for Krishgen quality, Long shelf-life, Expert technical support. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma, biological fluids and cell culture supernatant. The kit employs a double-antibody sandwich ELISA technique which leads to higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody.

SpeciesSummary :

Rat

Background :

Tumor necrosis factor-alpha (TNF-α), a homotrimeric 17 kDa protein, is a potent mediator of inflammatory and metabolic functions. TNF-α was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further, TNF-α has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF functions as a central mediator in many inflammatory and immunological processes. It can be induced by various products of microorganisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the p55 and p75 TNF-receptors. The receptors differ strongly in their intracellular signaling pathways.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

31.25 pg/ml to 2000 pg/ml

Sensitivity :

31 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

TNF Alpha

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage