GENLISA™ Mouse TNF Alpha ELISA | Krishgen Biosystems KB2145 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Mouse TNF Alpha ELISA

catalog :

KB2145

quantity :

1 x 96 wells

price :

270 USD

more info or order :

Krishgen Biosystems product webpage

citations: 15

Reference
Kamdi S, Raval A, Nakhate K. Phloridzin attenuates lipopolysaccharide-induced cognitive impairment via antioxidant, anti-inflammatory and neuromodulatory activities. Cytokine. 2021;139:155408 pubmed publisher
Singh L, Kaur A, Garg S, Bhatti R. Skimmetin/osthole mitigates pain-depression dyad via inhibiting inflammatory and oxidative stress-mediated neurotransmitter dysregulation. Metab Brain Dis. 2021;36:111-121 pubmed publisher
Ingawale D, Mandlik S, Patel S. Combination of Sarsasapogenin and Fluticasone attenuates ovalbumin-induced airway inflammation in a mouse asthma model. Immunopharmacol Immunotoxicol. 2020;42:128-137 pubmed publisher
Ingawale D, Mandlik S, Patel S. Anti-inflammatory potential of hecogenin on atopic dermatitis and airway hyper-responsiveness by regulation of pro-inflammatory cytokines. Immunopharmacol Immunotoxicol. 2019;41:327-336 pubmed publisher
Singh G, Kaur A, Kaur J, Bhatti M, Singh P, Bhatti R. Bergapten inhibits chemically induced nociceptive behavior and inflammation in mice by decreasing the expression of spinal PARP, iNOS, COX-2 and inflammatory cytokines. Inflammopharmacology. 2019;: pubmed publisher
Zameer S, Kaundal M, Vohora D, Ali J, Kalam Najmi A, Akhtar M. Ameliorative effect of alendronate against intracerebroventricular streptozotocin induced alteration in neurobehavioral, neuroinflammation and biochemical parameters with emphasis on Aβ and BACE-1. Neurotoxicology. 2019;70:122-134 pubmed publisher
Iqubal A, Sharma S, Sharma K, Bhavsar A, Hussain I, Iqubal M, et al. Intranasally administered pitavastatin ameliorates pentylenetetrazol-induced neuroinflammation, oxidative stress and cognitive dysfunction. Life Sci. 2018;211:172-181 pubmed publisher
Singh G, Bhatti R, Mannan R, Singh D, Kesavan A, Singh P. Osthole ameliorates neurogenic and inflammatory hyperalgesia by modulation of iNOS, COX-2, and inflammatory cytokines in mice. Inflammopharmacology. 2018;: pubmed publisher
Chowdhury A, Gawali N, Shinde P, Munshi R, Juvekar A. Imperatorin ameliorates lipopolysaccharide induced memory deficit by mitigating proinflammatory cytokines, oxidative stress and modulating brain-derived neurotropic factor. Cytokine. 2018;110:78-86 pubmed publisher
Ingawale D, Patel S. Hecogenin exhibits anti-arthritic activity in rats through suppression of pro-inflammatory cytokines in Complete Freund's adjuvant-induced arthritis. Immunopharmacol Immunotoxicol. 2017;:1-13 pubmed publisher
Patel N, Khan M, Bhutani K. Investigations on Leucas cephalotes (Roth.) Spreng. for inhibition of LPS-induced pro-inflammatory mediators in murine macrophages and in rat model. EXCLI J. 2015;14:508-16 pubmed publisher
Patel N, Bhutani K. Suppressive effects of Mimosa pudica (L.) constituents on the production of LPS-induced pro-inflammatory mediators. EXCLI J. 2014;13:1011-21 pubmed
Patel N, Ramandeep -, Bhutani K. Activity-guided fractionation of Ipomea fistulosa leaves for pro-inflammatory cytokines and nitric oxide inhibitory constituents. Nat Prod Commun. 2014;9:1749-50 pubmed
Sandeep Varma R, Shamsia S, Thiyagarajan O, Vidyashankar S, Patki P. Yashada bhasma (Zinc calx) and Tankana (Borax) inhibit Propionibacterium acne and suppresses acne induced inflammation in vitro. Int J Cosmet Sci. 2014;36:361-8 pubmed publisher
Patel N, Bhutani K. Pinostrobin and Cajanus lactone isolated from Cajanus cajan (L.) leaves inhibits TNF-α and IL-1β production: in vitro and in vivo experimentation. Phytomedicine. 2014;21:946-53 pubmed publisher

product information

Catalog Number :

KB2145

Product Name :

GENLISA™ Mouse TNF Alpha ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

270 USD

Product Description :

This ELISA is intended for the quantitative detection of TNF-Alpha in mouse serum, cell cuture supernatant and plasma samples. ELISA Features include:- Ready to use protocol, Standardisation and High Reproducibility, Lot to Lot Consistency, Accuracy and Precision. Validated against seven points for a Gold Ring Standard Quality ELISA - the benchmark sign for Krishgen quality, Long shelf-life, Expert technical support. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma, biological fluids and cell culture supernatant.

SpeciesSummary :

Mouse

Background :

Tumor Necrosis Factor-α (TNF- α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. Mouse TNF-α is a 17.5 KD protein containing 156 amino acid residues.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

3.5 pg/ml to 450 pg/ml

Sensitivity :

2.5 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

TNF Alpha

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage