GENLISA™ Mouse IL - 1 beta ELISA | Krishgen Biosystems KB2063 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Mouse IL - 1 beta ELISA

catalog :

KB2063

quantity :

1 x 96 wells

price :

270 USD

more info or order :

Krishgen Biosystems product webpage

citations: 23

Reference
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Srinivasan A, Aruldhas J, Perumal S, Ekambaram S. Phenolic acid bound arabinoxylans extracted from Little and Kodo millets modulate immune system mediators and pathways in RAW 264.7 cells. J Food Biochem. 2021;45:e13563 pubmed publisher
Singla E, Puri G, Dharwal V, Naura A. Gallic acid ameliorates COPD-associated exacerbation in mice. Mol Cell Biochem. 2021;476:293-302 pubmed publisher
Kaur A, Singh L, Garg S, Kaur H, Singh N, Bhatti R. Involvement of Oxidative Stress and Nerve Growth Factor in Behavioral and Biochemical Deficits of Experimentally Induced Musculoskeletal Pain in Mice: Ameliorative Effects of Heraclin. J Mol Neurosci. 2021;71:347-357 pubmed publisher
Iqubal A, Syed M, Haque M, Najmi A, Ali J, Haque S. Effect of nerolidol on cyclophosphamide-induced bone marrow and hematologic toxicity in Swiss albino mice. Exp Hematol. 2020;82:24-32 pubmed publisher
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Ekambaram S, Perumal S, Erusappan T, Srinivasan A. Hydrolysable tannin-rich fraction from Terminalia chebula Retz. fruits ameliorates collagen-induced arthritis in BALB/c mice. Inflammopharmacology. 2020;28:275-287 pubmed publisher
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Kaur A, Singh L, Singh N, Bhatti M, Bhatti R. Ameliorative effect of imperatorin in chemically induced fibromyalgia: Role of NMDA/NFkB mediated downstream signaling. Biochem Pharmacol. 2019;166:56-69 pubmed publisher
Iqbal B, Ali J, Ganguli M, Mishra S, Baboota S. Silymarin-loaded nanostructured lipid carrier gel for the treatment of skin cancer. Nanomedicine (Lond). 2019;14:1077-1093 pubmed publisher
Zameer S, Kaundal M, Vohora D, Ali J, Kalam Najmi A, Akhtar M. Ameliorative effect of alendronate against intracerebroventricular streptozotocin induced alteration in neurobehavioral, neuroinflammation and biochemical parameters with emphasis on Aβ and BACE-1. Neurotoxicology. 2019;70:122-134 pubmed publisher
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Singh T, Kaur T, Goel R. Ferulic Acid Supplementation for Management of Depression in Epilepsy. Neurochem Res. 2017;42:2940-2948 pubmed publisher
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product information

Catalog Number :

KB2063

Product Name :

GENLISA™ Mouse IL - 1 beta ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

270 USD

Product Description :

This ELISA is intended for the quantitative detection of IL-1 beta in mouse serum, cell cuture supernatant and plasma samples. ELISA Features include:- Ready to use protocol, Standardisation and High Reproducibility, Lot to Lot Consistency, Accuracy and Precision. Validated against seven points for a Gold Ring Standard Quality ELISA - the benchmark sign for Krishgen quality, Long shelf-life, Expert technical support. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma, biological fluids and cell culture supernatant. The kit employs a double-antibody sandwich ELISA technique which leads to higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody.

SpeciesSummary :

Mouse

Background :

Mouse IL-1β, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), mononuclear cell factor (MCF), is a ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1β is mostly cell associated with 23% amino acid homology with IL-1α. The immune regulatory role of IL-1β is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

15.63 pg/ml to 1000 pg/ml

Sensitivity :

15 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

IL - 1 beta

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage