GENLISA™ Mouse IFN-Gamma ELISA | Krishgen Biosystems KB2011 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Mouse IFN-Gamma ELISA

catalog :

KB2011

quantity :

1 x 96 wells

price :

270 USD

more info or order :

Krishgen Biosystems product webpage

citations: 6

Reference
Katoch P, Bisht G, Shrivastava R. In vivo infection and In vitro stress survival studies of acid susceptible mutant of Mycobacterium fortuitum. Int J Mycobacteriol. 2019;8:390-396 pubmed publisher
Malhotra P, Gupta A, Singh D, Mishra S, Singh S, Kumar R. Protection to immune system of mice by N-acetyl tryptophan glucoside (NATG) against gamma radiation induced immune suppression. Mol Immunol. 2019;114:578-590 pubmed publisher
Sharma Y, Ahmad A, Yavvari P, Kumar Muwal S, Bajaj A, Khan F. Targeted SHP-1 Silencing Modulates the Macrophage Phenotype, Leading to Metabolic Improvement in Dietary Obese Mice. Mol Ther Nucleic Acids. 2019;16:626-636 pubmed publisher
Nigam K, Kaur A, Tyagi A, Nematullah M, Khan F, Gabrani R, et al. Nose-to-brain delivery of lamotrigine-loaded PLGA nanoparticles. Drug Deliv Transl Res. 2019;: pubmed publisher
Gandhi M, Bhatt P, Chauhan G, Gupta S, Misra A, Mashru R. IGF-II-Conjugated Nanocarrier for Brain-Targeted Delivery of p11 Gene for Depression. AAPS PharmSciTech. 2019;20:50 pubmed publisher
Sharma Y, Bashir S, Ansarullah -, Faraz Khan M, Ahmad A, Khan F. Inhibition of Src homology 2 domain containing protein tyrosine phosphatase as the possible mechanism of metformin-assisted amelioration of obesity induced insulin resistance in high fat diet fed C57BL/6J mice. Biochem Biophys Res Commun. 2017;487:54-61 pubmed publisher

product information

Catalog Number :

KB2011

Product Name :

GENLISA™ Mouse IFN-Gamma ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

270 USD

Product Description :

This ELISA is intended for the quantitative detection of IFN Gamma in mouse serum, cell culture supernatant and plasma samples. ELISA Features include:- Ready to use protocol, Standardisation and High Reproducibility, Lot to Lot Consistency, Accuracy and Precision. Validated against seven points for a Gold Ring Standard Quality ELISA - the benchmark sign for Krishgen quality, Long shelf-life, Expert technical support. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma, biological fluids and cell culture supernatant. The kit employs a double-antibody sandwich ELISA technique which leads to higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody.

SpeciesSummary :

Mouse

Background :

The recombinant Mouse IFN-γ, a ~20 kDa factor is produced by activated T, B and NK cells and an anti-viral and anti-parasitic cytokine. IFN-γ inhibits proliferation of normal and transformed cells in synergy with other cytokines such as TNF-alpha. Immuno-modulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2 - 8 Deg C / dry ice at -20 Deg C

Calibration Range :

62.5 pg/ml to 2000 pg/ml

Sensitivity :

30 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

IFN-Gamma

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage