GENLISA™ Human VEGF ELISA | Krishgen Biosystems KB1155 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Human VEGF ELISA

catalog :

KB1155

quantity :

1 x 96 wells

price :

320 USD

more info or order :

Krishgen Biosystems product webpage

citations: 2

Reference
Boreak N, Khayrat N, Shami A, Zaylaee H, Hanbashi A, Souri S, et al. Metformin pre-conditioning enhances the angiogenic ability of the secretome of dental pulp stem cells. Saudi Pharm J. 2021;29:908-913 pubmed publisher
Aggarwal H, Nair J, Sharma P, Sehgal R, Naeem U, Rajora P, et al. Aegle marmelos differentially affects hepatic markers of glycolysis, insulin signalling pathway, hypoxia, and inflammation in HepG2 cells grown in fructose versus glucose-rich environment. Mol Cell Biochem. 2018;438:1-16 pubmed publisher

product information

Catalog Number :

KB1155

Product Name :

GENLISA™ Human VEGF ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

320 USD

Product Description :

This ELISA is intended for the quantitative detection of VEGF in human serum, cell culture supernatant and plasma samples. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

SpeciesSummary :

Human

Background :

The GENLISA™ ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

20 pg/ml – 320 pg/ml

Sensitivity :

18 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

VEGF

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage