GENLISA™ Human TNF-Alpha ELISA | Krishgen Biosystems KB1145 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Human TNF-Alpha ELISA

catalog :

KB1145

quantity :

1 x 96 wells

price :

240 USD

more info or order :

Krishgen Biosystems product webpage

citations: 25

Reference
Saikiran G, Mitra P, Sharma S, Kumar P, Sharma P. Selenium, oxidative stress and inflammatory markers in handicraft workers occupationally exposed to lead. Arch Environ Occup Health. 2022;77:561-567 pubmed publisher
Gupta K, Pandey S, Singh R, Kumari A, Sen P, Singh G. Roflumilast improves resolution of sepsis-induced acute kidney injury by retarding late phase renal interstitial immune cells infiltration and leakage in urinary sediments. Fundam Clin Pharmacol. 2022;36:114-132 pubmed publisher
Goyal T, Mitra P, Singh P, Ghosh R, Sharma S, Sharma P. Association of microRNA expression with changes in immune markers in workers with cadmium exposure. Chemosphere. 2021;274:129615 pubmed publisher
Pandey S, Rai N, Mahtab A, Mittal D, Ahmad F, Sandal N, et al. Hyaluronate-functionalized hydroxyapatite nanoparticles laden with methotrexate and teriflunomide for the treatment of rheumatoid arthritis. Int J Biol Macromol. 2021;171:502-513 pubmed publisher
Goyal T, Mitra P, Singh P, Sharma P, Sharma S. Evaluation of oxidative stress and pro-inflammatory cytokines in occupationally cadmium exposed workers. Work. 2021;69:67-73 pubmed publisher
Goyal T, Mitra P, Singh P, Ghosh R, Lingeswaran M, Sharma S, et al. Alterations in Th17 and Treg Lymphocyte Subset in Workers Occupationally Exposed to Lead. Biol Trace Elem Res. 2021;199:1693-1700 pubmed publisher
Vaghasiya K, Eram A, Sharma A, Ray E, Adlakha S, Verma R. Alginate Microspheres Elicit Innate M1-Inflammatory Response in Macrophages Leading to Bacillary Killing. AAPS PharmSciTech. 2019;20:241 pubmed publisher
Varma S, Sivaprakasam T, Arumugam I, Dilip N, Raghuraman M, Pavan K, et al. In vitro anti-inflammatory and skin protective properties of Virgin coconut oil. J Tradit Complement Med. 2019;9:5-14 pubmed publisher
Patel M, Patel B. Repurposing of sodium valproate in colon cancer associated with diabetes mellitus: Role of HDAC inhibition. Eur J Pharm Sci. 2018;121:188-199 pubmed publisher
Bakshi D, Kaur G, Singh D, Sahota J, Thakur A, Grover S. Estimation of Plasma Levels of Tumor Necrosis Factor-a, Interleukin-4 and 6 in Patients with Chronic Periodontitis and Type II Diabetes Mellitus. J Contemp Dent Pract. 2018;19:166-169 pubmed
Acharya A, Thakur S, Muddapur M, Kulkarni R. Tumor necrosis factor-α, interleukin-4 and -6 in the serum of health, chronic periodontitis, and type 2 diabetes mellitus. J Indian Soc Periodontol. 2016;20:509-513 pubmed publisher
Gupta K, Gupta R, Bhatia M, Tripathi A, Gupta L. Effect of Agomelatine and Fluoxetine on HAM-D Score, Serum Brain-Derived Neurotrophic Factor, and Tumor Necrosis Factor-α Level in Patients With Major Depressive Disorder With Severe Depression. J Clin Pharmacol. 2017;57:1519-1526 pubmed publisher
Aggarwal H, Nair J, Sharma P, Sehgal R, Naeem U, Rajora P, et al. Aegle marmelos differentially affects hepatic markers of glycolysis, insulin signalling pathway, hypoxia, and inflammation in HepG2 cells grown in fructose versus glucose-rich environment. Mol Cell Biochem. 2018;438:1-16 pubmed publisher
Kumar A, Saini V, Kumar A, Kaur J, Kaur J. Modulation of Trehalose Dimycolate and Immune System by Rv0774c Protein Enhanced the Intracellular Survival of Mycobacterium smegmatis in Human Macrophages Cell Line. Front Cell Infect Microbiol. 2017;7:289 pubmed publisher
Gupta R, Gupta K, Tripathi A, Bhatia M, Gupta L. Effect of Mirtazapine Treatment on Serum Levels of Brain-Derived Neurotrophic Factor and Tumor Necrosis Factor-α in Patients of Major Depressive Disorder with Severe Depression. Pharmacology. 2016;97:184-8 pubmed publisher
Dhiman S, Mishra N, Sharma S. Development of PEGylated solid lipid nanoparticles of pentoxifylline for their beneficial pharmacological potential in pathological cardiac hypertrophy. Artif Cells Nanomed Biotechnol. 2016;44:1901-1908 pubmed
Varma R, Guruprasad K, Satyamoorthy K, Kumar L, Babu U, Patki S. IM-133N modulates cytokine secretion by RAW264.7 and THP-1 cells. J Immunotoxicol. 2016;13:217-25 pubmed publisher
Sandeep Varma R, Shamsia S, Thiyagarajan O, Vidyashankar S, Patki P. Yashada bhasma (Zinc calx) and Tankana (Borax) inhibit Propionibacterium acne and suppresses acne induced inflammation in vitro. Int J Cosmet Sci. 2014;36:361-8 pubmed publisher
Fatima G, Mahdi A, Das S, Anjum B, Verma N, Kumar P, et al. Lack of Circadian Pattern of Serum TNF-α and IL-6 in Patients with Fibromyalgia Syndrome. Indian J Clin Biochem. 2012;27:340-3 pubmed publisher
Nankar S, Bajaj P, Sravanthi R, Pande A. Differential interaction of peptides derived from C-terminal domain of human apolipoprotein E with platelet activating factor analogs. Biochimie. 2013;95:1196-207 pubmed publisher
Vidyashankar S, Sandeep Varma R, Patki P. Quercetin ameliorate insulin resistance and up-regulates cellular antioxidants during oleic acid induced hepatic steatosis in HepG2 cells. Toxicol In Vitro. 2013;27:945-53 pubmed publisher
Nankar S, Pande A. Physicochemical properties of bacterial pro-inflammatory lipids influence their interaction with apolipoprotein-derived peptides. Biochim Biophys Acta. 2013;1831:853-62 pubmed publisher
Vidyashankar S, Sharath Kumar L, Barooah V, Sandeep Varma R, Nandakumar K, Patki P. Liv.52 up-regulates cellular antioxidants and increase glucose uptake to circumvent oleic acid induced hepatic steatosis in HepG2 cells. Phytomedicine. 2012;19:1156-65 pubmed publisher
Vidyashankar S, Nandakumar K, Patki P. Alcohol depletes coenzyme-Q(10) associated with increased TNF-alpha secretion to induce cytotoxicity in HepG2 cells. Toxicology. 2012;302:34-9 pubmed publisher
Sandeep Varma R, Ashok G, Vidyashankar S, Nandakumar K, Patki P. Bresol inhibits phosphodiesterase 4 gene expression and modulates the levels of select mediators of inflammation in human monocytic cells. J Immunotoxicol. 2011;8:315-23 pubmed publisher

product information

Catalog Number :

KB1145

Product Name :

GENLISA™ Human TNF-Alpha ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

240 USD

Product Description :

This ELISA is intended for the quantitative detection of TNF Alpha in human serum, cell culture supernatant and plasma samples. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

SpeciesSummary :

Human

Background :

Tumor Necrosis Factor-α (TNF- α) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF- α is a 17.4 kD protein containing 157 amino acid residues.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

7.8 pg/ml to 500 pg/ml

Sensitivity :

7 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

TNF Alpha

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage