GENLISA™ Human IL-10 ELISA | Krishgen Biosystems KB1072 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Human IL-10 ELISA

catalog :

KB1072

quantity :

1 x 96 wells

price :

240 USD

more info or order :

Krishgen Biosystems product webpage

citations: 7

Reference
Saikiran G, Mitra P, Sharma S, Kumar P, Sharma P. Selenium, oxidative stress and inflammatory markers in handicraft workers occupationally exposed to lead. Arch Environ Occup Health. 2022;77:561-567 pubmed publisher
Goyal T, Mitra P, Singh P, Ghosh R, Sharma S, Sharma P. Association of microRNA expression with changes in immune markers in workers with cadmium exposure. Chemosphere. 2021;274:129615 pubmed publisher
Goyal T, Mitra P, Singh P, Ghosh R, Lingeswaran M, Sharma S, et al. Estimation of lymphocyte subsets and cytokine levels in workers occupationally exposed to cadmium. J Trace Elem Med Biol. 2021;64:126681 pubmed publisher
Goyal T, Mitra P, Singh P, Ghosh R, Lingeswaran M, Sharma S, et al. Alterations in Th17 and Treg Lymphocyte Subset in Workers Occupationally Exposed to Lead. Biol Trace Elem Res. 2021;199:1693-1700 pubmed publisher
Kumar A, Singh S, Singh R, Kaur J. Rv0774c, an iron stress inducible, extracellular esterase is involved in immune-suppression associated with altered cytokine and TLR2 expression. Int J Med Microbiol. 2017;307:126-138 pubmed publisher
Acharya A, Thakur S, Muddapur M. Evaluation of serum interleukin-10 levels as a predictor of glycemic alteration in chronic periodontitis and type 2 diabetes mellitus. J Indian Soc Periodontol. 2015;19:388-92 pubmed publisher
Acharya A, Thakur S, Muddapur M. Effect of scaling and root planing on serum interleukin-10 levels and glycemic control in chronic periodontitis and type 2 diabetes mellitus. J Indian Soc Periodontol. 2015;19:188-93 pubmed publisher

product information

Catalog Number :

KB1072

Product Name :

GENLISA™ Human IL-10 ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

240 USD

Product Description :

This ELISA is intended for the quantitative detection of IL-10 in human serum, cell culture supernatant and plasma samples. ELISA Features include:- Ready to use protocol, Standardisation and High Reproducibility, Lot to Lot Consistency, Accuracy and Precision. Validated against seven points for a Gold Ring Standard Quality ELISA - the benchmark sign for Krishgen quality, Long shelf-life, Expert technical support. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma, biological fluids and cell culture supernatant. The kit employs a double-antibody sandwich ELISA technique which leads to higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody.

SpeciesSummary :

Human

Background :

Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an antiinflammatory cytokine. This cytokine is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

7.8 pg/ml to 500 pg/ml

Sensitivity :

7 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

IL-10

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage