GENLISA™ Human IL-8 ELISA | Krishgen Biosystems KB1070 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Human IL-8 ELISA

catalog :

KB1070

quantity :

1 x 96 wells

price :

240 USD

more info or order :

Krishgen Biosystems product webpage

citations: 6

Reference
Varma S, Sivaprakasam T, Arumugam I, Dilip N, Raghuraman M, Pavan K, et al. In vitro anti-inflammatory and skin protective properties of Virgin coconut oil. J Tradit Complement Med. 2019;9:5-14 pubmed publisher
Banerjee P, Malik A, Malhotra S, Gupta S. Role of STAT signaling and autocrine action of chemokines during H2 O 2 induced HTR-8/SVneo trophoblastic cells invasion. J Cell Physiol. 2019;234:1380-1397 pubmed publisher
Kumar A, Singh S, Singh R, Kaur J. Rv0774c, an iron stress inducible, extracellular esterase is involved in immune-suppression associated with altered cytokine and TLR2 expression. Int J Med Microbiol. 2017;307:126-138 pubmed publisher
Sandeep Varma R, Shamsia S, Thiyagarajan O, Vidyashankar S, Patki P. Yashada bhasma (Zinc calx) and Tankana (Borax) inhibit Propionibacterium acne and suppresses acne induced inflammation in vitro. Int J Cosmet Sci. 2014;36:361-8 pubmed publisher
Nankar S, Pande A. Properties of apolipoprotein E derived peptide modulate their lipid-binding capacity and influence their anti-inflammatory function. Biochim Biophys Acta. 2014;1841:620-9 pubmed publisher
Vidyashankar S, Sandeep Varma R, Patki P. Quercetin ameliorate insulin resistance and up-regulates cellular antioxidants during oleic acid induced hepatic steatosis in HepG2 cells. Toxicol In Vitro. 2013;27:945-53 pubmed publisher

product information

Catalog Number :

KB1070

Product Name :

GENLISA™ Human IL-8 ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

240 USD

Product Description :

This ELISA is intended for the quantitative detection of IL-8 in human serum, cell culture supernatant and plasma samples. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

SpeciesSummary :

Human

Background :

IL-8 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells.IL-8 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-8 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. Recombinant Human IL-8 is a 20.9 kDa protein containing 184 amino acids.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

15.62 pg/ml - 1000 pg/ml

Sensitivity :

15 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

IL-8

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage