GENLISA™ Human IL-6 ELISA | Krishgen Biosystems KB1068 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Human IL-6 ELISA

catalog :

KB1068

quantity :

1 x 96 wells

price :

240 USD

more info or order :

Krishgen Biosystems product webpage

citations: 13

Reference
Saikiran G, Mitra P, Sharma S, Kumar P, Sharma P. Selenium, oxidative stress and inflammatory markers in handicraft workers occupationally exposed to lead. Arch Environ Occup Health. 2022;77:561-567 pubmed publisher
Thambi M, Nathan J, Bailur S, Unnikrishnan M, Ballal M, Radhakrishnan K. Is the antiseizure effect of ketogenic diet in children with drug-resistant epilepsy mediated through proinflammatory cytokines?. Epilepsy Res. 2021;176:106724 pubmed publisher
Goyal T, Mitra P, Singh P, Ghosh R, Sharma S, Sharma P. Association of microRNA expression with changes in immune markers in workers with cadmium exposure. Chemosphere. 2021;274:129615 pubmed publisher
Pandey S, Rai N, Mahtab A, Mittal D, Ahmad F, Sandal N, et al. Hyaluronate-functionalized hydroxyapatite nanoparticles laden with methotrexate and teriflunomide for the treatment of rheumatoid arthritis. Int J Biol Macromol. 2021;171:502-513 pubmed publisher
Goyal T, Mitra P, Singh P, Ghosh R, Lingeswaran M, Sharma S, et al. Alterations in Th17 and Treg Lymphocyte Subset in Workers Occupationally Exposed to Lead. Biol Trace Elem Res. 2021;199:1693-1700 pubmed publisher
Vaghasiya K, Eram A, Sharma A, Ray E, Adlakha S, Verma R. Alginate Microspheres Elicit Innate M1-Inflammatory Response in Macrophages Leading to Bacillary Killing. AAPS PharmSciTech. 2019;20:241 pubmed publisher
Varma S, Sivaprakasam T, Arumugam I, Dilip N, Raghuraman M, Pavan K, et al. In vitro anti-inflammatory and skin protective properties of Virgin coconut oil. J Tradit Complement Med. 2019;9:5-14 pubmed publisher
Bakshi D, Kaur G, Singh D, Sahota J, Thakur A, Grover S. Estimation of Plasma Levels of Tumor Necrosis Factor-a, Interleukin-4 and 6 in Patients with Chronic Periodontitis and Type II Diabetes Mellitus. J Contemp Dent Pract. 2018;19:166-169 pubmed
Acharya A, Thakur S, Muddapur M, Kulkarni R. Tumor necrosis factor-α, interleukin-4 and -6 in the serum of health, chronic periodontitis, and type 2 diabetes mellitus. J Indian Soc Periodontol. 2016;20:509-513 pubmed publisher
Jaiswal S, Mishra P. Antimicrobial and antibiofilm activity of curcumin-silver nanoparticles with improved stability and selective toxicity to bacteria over mammalian cells. Med Microbiol Immunol. 2018;207:39-53 pubmed publisher
Khan M, Garg K, Bhurani D, Agarwal N. Early manifestation of mild cognitive impairment in B-cell non-Hodgkin's lymphoma patients receiving CHOP and rituximab-CHOP chemotherapy. Naunyn Schmiedebergs Arch Pharmacol. 2016;389:1253-1265 pubmed
Kobawala T, Trivedi T, Gajjar K, Patel D, Patel G, Ghosh N. Significance of Interleukin-6 in Papillary Thyroid Carcinoma. J Thyroid Res. 2016;2016:6178921 pubmed publisher
Fatima G, Mahdi A, Das S, Anjum B, Verma N, Kumar P, et al. Lack of Circadian Pattern of Serum TNF-α and IL-6 in Patients with Fibromyalgia Syndrome. Indian J Clin Biochem. 2012;27:340-3 pubmed publisher

product information

Catalog Number :

KB1068

Product Name :

GENLISA™ Human IL-6 ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

240 USD

Product Description :

This ELISA is intended for the quantitative detection of IL-6 in human serum, cell culture supernatant and plasma samples. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

SpeciesSummary :

Human

Background :

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. Recombinant human IL-6 is a 20.9 kD protein containing 184 amino acids. Interleukin-6 (IL-6) is a member of the IL-6 family. It consists of polypeptide cytokines with a four -helix structure and a molecular mass of 21 to 28 kDa.It is a pleiotropic, pro-inflammatory cytokine produced by a variety of cell types, including lymphocytes, monocytes, and fibroblasts. IL-6 Stimulates acute phase reactions. These promote the activation of innate immunity, keeping tissue damage at bay. It also helps T-cells to differentiate early in their development. It is required for progenitor cell development, and also for T-cell and NK cell activation. In addition, it helps them to achieve pathogen lysis inside the cells.Interleukin-6 also helps B-cells differentiate and proliferate, and promotes the formation of plasma cells from B-cells. In addition, as a growth factor for these cells, it enhances antibody release, in the form of IgA and IgG.Interleukin-6 is also vital for the development of blood cells, whether white cells, red cells or platelets. It seems to play an important role in the development of Kaposi s sarcoma, and multiple myeloma.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

6.25 pg/ml to 400 pg/ml

Sensitivity :

5 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

IL-6

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage