GENLISA™ Human IL-4 ELISA | Krishgen Biosystems KB1066 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Human IL-4 ELISA

catalog :

KB1066

quantity :

1 x 96 wells

price :

240 USD

more info or order :

Krishgen Biosystems product webpage

citations: 4

Reference
Goyal T, Mitra P, Singh P, Ghosh R, Sharma S, Sharma P. Association of microRNA expression with changes in immune markers in workers with cadmium exposure. Chemosphere. 2021;274:129615 pubmed publisher
Goyal T, Mitra P, Singh P, Ghosh R, Lingeswaran M, Sharma S, et al. Alterations in Th17 and Treg Lymphocyte Subset in Workers Occupationally Exposed to Lead. Biol Trace Elem Res. 2021;199:1693-1700 pubmed publisher
Bakshi D, Kaur G, Singh D, Sahota J, Thakur A, Grover S. Estimation of Plasma Levels of Tumor Necrosis Factor-a, Interleukin-4 and 6 in Patients with Chronic Periodontitis and Type II Diabetes Mellitus. J Contemp Dent Pract. 2018;19:166-169 pubmed
Acharya A, Thakur S, Muddapur M, Kulkarni R. Tumor necrosis factor-α, interleukin-4 and -6 in the serum of health, chronic periodontitis, and type 2 diabetes mellitus. J Indian Soc Periodontol. 2016;20:509-513 pubmed publisher

product information

Catalog Number :

KB1066

Product Name :

GENLISA™ Human IL-4 ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

240 USD

Product Description :

This ELISA is intended for the quantitative detection of IL-4 in human serum, cell culture supernatant and plasma samples. ELISA Features include:- Ready to use protocol, Standardisation and High Reproducibility, Lot to Lot Consistency, Accuracy and Precision. Validated against seven points for a Gold Ring Standard Quality ELISA - the benchmark sign for Krishgen quality, Long shelf-life, Expert technical support. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma, biological fluids and cell culture supernatant. The kit employs a double-antibody sandwich ELISA technique which leads to higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody.

SpeciesSummary :

Human

Background :

Interleukin-4, abbreviated IL-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humeral and adaptive immunity. IL-4 induces B-cell class switching to Ice, and up-regulates MHC class II production. Overproduction of IL-4 is associated with allergies.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

31.3-2000pg/ml

Sensitivity :

31.3 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

IL-4

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage