ELISA/assay

GENLISA™ Human IL-1 Beta ELISA

KB1063

1 x 96 wells

240 USD

GENLISA™ Human IL-1 Beta ELISA

ELISA

1 x 96 wells

240 USD

This ELISA is intended for the quantitative detection of IL-1 beta in human serum, cell culture supernatant and plasma samples. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

Human

IL-1β is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-1β plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-1β is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. Recombinant human IL-1 β is a 20.9 kD protein containing 184 amino acids.

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

2-8 Deg C

3.91 - 250 pg/ml

3 pg/ml

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

IL-1 Beta

RUO

2-8 degrees C