Anti-DNA-RNA Hybrid [S9.6] Antibody, 1mg (10x100ug) | KeraFAST ENH002 product information

product summary

company name :

KeraFAST

product type :

antibody

product name :

Anti-DNA-RNA Hybrid [S9.6] Antibody, 1mg (10x100ug)

catalog :

ENH002

quantity :

1 mg (10 x100 ug)

price :

2,750.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S9.6

more info or order :

KeraFAST product webpage

product information

Antigen: :

S9.6 ΦX174 bacteriophage-derived synthetic DNA/RNA antigen

Buffer: :

0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide

Clonality: :

Monoclonal

Clone Name: :

S9.6

Immunogen: :

ΦX174 bacteriophage-derived synthetic DNA/RNA

Isotype: :

IgG2a

Paratope: :

DYYGSRWFDY (proposed)

Product Type: :

Antibody

Purification Method: :

Protein G

Reactivity: :

High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids

Shipped: :

Cold packs

Species Immunized: :

mouse

Storage: :

-20C

Tested Applications: :

Dot Blot Analysis: 0.2 µg/mL. Affinity Binding Assay: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected increased DNA RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected R-loops formed over beta-actin gene using HeLa chromatin preparation. RNase H treatment of the chromatin preparation prevented clone S9.6 from immunoprecipitating target chromatin fragments (Skourti-Stathaki, K., et al. (2011). Mol. Cell. 42(6):794-805). Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast by ChIP-seq analysis (El Hage, A., et al. (2014). PLoS Genet. 10(10):e1004716). Immunocytochemistry Analysis: Representative lots immunolocalized nuclear R loops by fluorescent immunocytochemistry staining of methanol-fixed H1 human embryonic stem cells (hESCs) and formaldehyde-fixed HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365; Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825). Immunoprecipitation Analysis: A representative lot immunoprecipitated in vitro transcribed R-loop substrate (DNA-RNA hybrid), but not doouble-stranded DNA (dsDNA) (Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825). See also: S9.6 Publications by Application

Catalog Number :

ENH002

Product Name :

Anti-DNA-RNA Hybrid [S9.6] Antibody, 1mg (10x100ug)

Price :

2,750.00 USD

Size :

1 mg (10 x100 ug)

more info or order :

KeraFAST product webpage