product summary
company name :
KeraFAST
product type :
antibody
product name :
Anti-DNA-RNA Hybrid [S9.6] Antibody, 100ug
catalog :
ENH001
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S9.6
reactivity :
fission yeast, fruit fly
application :
western blot, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, RNA immunoprecipitation, other
more info or order :
KeraFAST product webpage
citations: 33
Published Application/Species/Sample/DilutionReference
  • other; fruit fly ; 1:500; loading ...; fig 4f
Jiang Y, Lin S, Chen J, Tsai H, Hsieh T, Fu C. Electron tomographic analysis reveals ultrastructural features of mitochondrial cristae architecture which reflect energetic state and aging. Sci Rep. 2017;7:45474 pubmed publisher
  • chromatin immunoprecipitation; fission yeast; loading ...; fig 2a
  • western blot; fission yeast; loading ...; fig 2a
Ohle C, Tesorero R, Schermann G, Dobrev N, Sinning I, Fischer T. Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair. Cell. 2016;167:1001-1013.e7 pubmed publisher
Wang Y, Ma B, Liu X, Gao G, Che Z, Fan M, et al. ZFP281-BRCA2 prevents R-loop accumulation during DNA replication. Nat Commun. 2022;13:3493 pubmed publisher
Buemi V, Schillaci O, Santorsola M, Bonazza D, Broccia P, Zappone A, et al. TGS1 mediates 2,2,7-trimethyl guanosine capping of the human telomerase RNA to direct telomerase dependent telomere maintenance. Nat Commun. 2022;13:2302 pubmed publisher
Lappin K, Barros E, Jhujh S, Irwin G, McMillan H, Liberante F, et al. Cancer-Associated SF3B1 Mutations Confer a BRCA-Like Cellular Phenotype and Synthetic Lethality to PARP Inhibitors. Cancer Res. 2022;82:819-830 pubmed publisher
Kang H, Eom H, Kim H, Myung K, Kwon H, Choi J. Thrap3 promotes R-loop resolution via interaction with methylated DDX5. Exp Mol Med. 2021;53:1602-1611 pubmed publisher
Gartrell J, Mellado Largarde M, Clay M, Bahrami A, Sahr N, Sykes A, et al. SLFN11 is Widely Expressed in Pediatric Sarcoma and Induces Variable Sensitization to Replicative Stress Caused By DNA-Damaging Agents. Mol Cancer Ther. 2021;20:2151-2165 pubmed publisher
Park S, Bennett Baker P, Ahmed S, Arlt M, Ljungman M, Glover T, et al. Locus-specific transcription silencing at the FHIT gene suppresses replication stress-induced copy number variant formation and associated replication delay. Nucleic Acids Res. 2021;49:7507-7524 pubmed publisher
Wu J, Wu C, Xing F, Cao L, Zeng W, Guo L, et al. Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells. Cell Res. 2021;: pubmed publisher
van der Weegen Y, de Lint K, van den Heuvel D, Nakazawa Y, Mevissen T, van Schie J, et al. ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation. Nat Cell Biol. 2021;23:595-607 pubmed publisher
Safari M, Litman T, Robey R, Aguilera A, Chakraborty A, Reinhold W, et al. R-Loop-Mediated ssDNA Breaks Accumulate Following Short-Term Exposure to the HDAC Inhibitor Romidepsin. Mol Cancer Res. 2021;19:1361-1374 pubmed publisher
Park K, Ryoo J, Jeong H, Kim M, Lee S, Hwang S, et al. Aicardi-Goutières syndrome-associated gene SAMHD1 preserves genome integrity by preventing R-loop formation at transcription-replication conflict regions. PLoS Genet. 2021;17:e1009523 pubmed publisher
Kim C, Sung S, Kim J, Lee H, Jung Y, Shin S, et al. Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells. Nat Commun. 2021;12:1097 pubmed publisher
Patel P, Abraham K, Guturi K, Halaby M, Khan Z, Palomero L, et al. RNF168 regulates R-loop resolution and genomic stability in BRCA1/2-deficient tumors. J Clin Invest. 2021;131: pubmed publisher
Chakraborty A, Jenjaroenpun P, Li J, El Hilali S, McCulley A, Haarer B, et al. Replication Stress Induces Global Chromosome Breakage in the Fragile X Genome. Cell Rep. 2020;32:108179 pubmed publisher
Trembinski D, Bink D, Theodorou K, Sommer J, Fischer A, van Bergen A, et al. Aging-regulated anti-apoptotic long non-coding RNA Sarrah augments recovery from acute myocardial infarction. Nat Commun. 2020;11:2039 pubmed publisher
Chang W, Xu J, Lin T, Hsu J, Hsieh Li H, Hwu Y, et al. Survival Motor Neuron Protein Participates in Mouse Germ Cell Development and Spermatogonium Maintenance. Int J Mol Sci. 2020;21: pubmed publisher
Chappidi N, Nascakova Z, Boleslavska B, Zellweger R, Isik E, Andrs M, et al. Fork Cleavage-Religation Cycle and Active Transcription Mediate Replication Restart after Fork Stalling at Co-transcriptional R-Loops. Mol Cell. 2019;: pubmed publisher
Argaud D, Boulanger M, Chignon A, Mkannez G, Mathieu P. Enhancer-mediated enrichment of interacting JMJD3-DDX21 to ENPP2 locus prevents R-loop formation and promotes transcription. Nucleic Acids Res. 2019;: pubmed publisher
Alfano L, Caporaso A, Altieri A, Dell Aquila M, Landi C, Bini L, et al. Depletion of the RNA binding protein HNRNPD impairs homologous recombination by inhibiting DNA-end resection and inducing R-loop accumulation. Nucleic Acids Res. 2019;47:4068-4085 pubmed publisher
Landsverk H, Sandquist L, Sridhara S, R dland G, Sabino J, de Almeida S, et al. Regulation of ATR activity via the RNA polymerase II associated factors CDC73 and PNUTS-PP1. Nucleic Acids Res. 2019;47:1797-1813 pubmed publisher
Bhatia V, Valdés Sánchez L, Rodríguez Martínez D, Bhattacharya S. Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice. F1000Res. 2018;7:1233 pubmed publisher
Teng Y, Yadav T, Duan M, Tan J, Xiang Y, Gao B, et al. ROS-induced R loops trigger a transcription-coupled but BRCA1/2-independent homologous recombination pathway through CSB. Nat Commun. 2018;9:4115 pubmed publisher
Andrews A, McCartney H, Errington T, D Andrea A, Macara I. A senataxin-associated exonuclease SAN1 is required for resistance to DNA interstrand cross-links. Nat Commun. 2018;9:2592 pubmed publisher
Lu W, Hawley B, Skalka G, Baldock R, Smith E, Bader A, et al. Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair. Nat Commun. 2018;9:532 pubmed publisher
Chen L, Chen J, Huang Y, Gu Y, Qiu J, Qian H, et al. The Augmented R-Loop Is a Unifying Mechanism for Myelodysplastic Syndromes Induced by High-Risk Splicing Factor Mutations. Mol Cell. 2018;69:412-425.e6 pubmed publisher
Komseli E, Pateras I, Krejsgaard T, Stawiski K, Rizou S, Polyzos A, et al. A prototypical non-malignant epithelial model to study genome dynamics and concurrently monitor micro-RNAs and proteins in situ during oncogene-induced senescence. BMC Genomics. 2018;19:37 pubmed publisher
Choi J, Hwang S, Ahn K. Interplay between RNASEH2 and MOV10 controls LINE-1 retrotransposition. Nucleic Acids Res. 2018;46:1912-1926 pubmed publisher
Chang E, Novoa C, Aristizabal M, Coulombe Y, Segovia R, Chaturvedi R, et al. RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability. J Cell Biol. 2017;216:3991-4005 pubmed publisher
Jones S, Fleuren E, Frankum J, Konde A, Williamson C, Krastev D, et al. ATR Is a Therapeutic Target in Synovial Sarcoma. Cancer Res. 2017;77:7014-7026 pubmed publisher
Walker C, Herranz Martin S, Karyka E, Liao C, Lewis K, Elsayed W, et al. C9orf72 expansion disrupts ATM-mediated chromosomal break repair. Nat Neurosci. 2017;20:1225-1235 pubmed publisher
Jangi M, Fleet C, Cullen P, Gupta S, Mekhoubad S, Chiao E, et al. SMN deficiency in severe models of spinal muscular atrophy causes widespread intron retention and DNA damage. Proc Natl Acad Sci U S A. 2017;114:E2347-E2356 pubmed publisher
Sagie S, Toubiana S, Hartono S, Katzir H, Tzur Gilat A, Havazelet S, et al. Telomeres in ICF syndrome cells are vulnerable to DNA damage due to elevated DNA:RNA hybrids. Nat Commun. 2017;8:14015 pubmed publisher
product information
Antigen: :
S9.6 ΦX174 bacteriophage-derived synthetic DNA/RNA antigen
Buffer: :
0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Clonality: :
Monoclonal
Clone Name: :
S9.6
Immunogen: :
ΦX174 bacteriophage-derived synthetic DNA/RNA
Isotype: :
IgG2a
Paratope: :
DYYGSRWFDY (proposed)
Product Type: :
Antibody
Purification Method: :
Protein G
Reactivity: :
High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids
Shipped: :
Cold packs
Species Immunized: :
mouse
Storage: :
-20C
Tested Applications: :
Dot Blot Analysis: 0.2 µg/mL. Affinity Binding Assay: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected increased DNA RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected R-loops formed over beta-actin gene using HeLa chromatin preparation. RNase H treatment of the chromatin preparation prevented clone S9.6 from immunoprecipitating target chromatin fragments (Skourti-Stathaki, K., et al. (2011). Mol. Cell. 42(6):794-805). Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast by ChIP-seq analysis (El Hage, A., et al. (2014). PLoS Genet. 10(10):e1004716). Immunocytochemistry Analysis: Representative lots immunolocalized nuclear R loops by fluorescent immunocytochemistry staining of methanol-fixed H1 human embryonic stem cells (hESCs) and formaldehyde-fixed HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365; Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825). Immunoprecipitation Analysis: A representative lot immunoprecipitated in vitro transcribed R-loop substrate (DNA-RNA hybrid), but not doouble-stranded DNA (dsDNA) (Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825). See also: S9.6 Publications by Application
Catalog Number :
ENH001
Product Name :
Anti-DNA-RNA Hybrid [S9.6] Antibody, 100ug
Size :
100 ug
more info or order :
KeraFAST product webpage
company information
KeraFAST
CustomerService@kerafast.com
http://www.kerafast.com