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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
TCR V beta F1 Monoclonal Antibody (8A3)
catalog :
TCR1151
quantity :
200 ug
price :
US 491.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8A3
reactivity :
fission yeast, human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 69
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; loading ...; fig 3d
Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
  • immunohistochemistry - paraffin section; human; tbl 1
Miyata Takata T, Takata K, Kato S, Hu L, Noujima Harada M, Chuang S, et al. Clinicopathological analysis of primary central nervous system NK/T cell lymphoma: rare and localized aggressive tumour among extranasal NK/T cell tumours. Histopathology. 2017;71:287-295 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 1a
Fuchs T, Hahn M, Riabov V, Yin S, Kzhyshkowska J, Busch S, et al. A combinatorial αβ T cell receptor expressed by macrophages in the tumor microenvironment. Immunobiology. 2017;222:39-44 pubmed publisher
  • immunohistochemistry; human; 1:100; fig 3
Louie C, DiMaio M, Matsukuma K, Coutre S, Berry G, Longacre T. Idelalisib-associated Enterocolitis: Clinicopathologic Features and Distinction From Other Enterocolitides. Am J Surg Pathol. 2015;39:1653-60 pubmed publisher
  • immunoprecipitation; human; fig 1
Feige M, Behnke J, Mittag T, Hendershot L. Dimerization-dependent folding underlies assembly control of the clonotypic αβT cell receptor chains. J Biol Chem. 2015;290:26821-31 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; fig 3
Takata K, Noujima Harada M, Miyata Takata T, Ichimura K, Sato Y, Miyata T, et al. Clinicopathologic analysis of 6 lymphomatoid gastropathy cases: expanding the disease spectrum to CD4-CD8+ cases. Am J Surg Pathol. 2015;39:1259-66 pubmed publisher
  • immunohistochemistry - paraffin section; human
Jeon Y, Kim J, Sung J, Han J, Ko Y. Epstein-Barr virus-positive nodal T/NK-cell lymphoma: an analysis of 15 cases with distinct clinicopathological features. Hum Pathol. 2015;46:981-90 pubmed publisher
  • immunohistochemistry - paraffin section; human
Wang L, Wang G, Gao T. Acneiform primary cutaneous CD4-positive small/medium pleomorphic T-cell lymphoma with prominent necrosis. J Cutan Pathol. 2015;42:265-70 pubmed publisher
  • immunohistochemistry; human; 1:100
Lester L, Ewalt M, Warnke R, Kim J. Systemic panniculitis-like T-cell lymphoma with involvement of mesenteric fat and subcutis. J Cutan Pathol. 2015;42:46-9 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Takata K, Hong M, Sitthinamsuwan P, Loong F, Tan S, Liau J, et al. Primary cutaneous NK/T-cell lymphoma, nasal type and CD56-positive peripheral T-cell lymphoma: a cellular lineage and clinicopathologic study of 60 patients from Asia. Am J Surg Pathol. 2015;39:1-12 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Yamaguchi M, Takata K, Yoshino T, Ishizuka N, Oguchi M, Kobayashi Y, et al. Prognostic biomarkers in patients with localized natural killer/T-cell lymphoma treated with concurrent chemoradiotherapy. Cancer Sci. 2014;105:1435-41 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:40
Onaindia A, Montes Moreno S, Rodriguez Pinilla S, Batlle A, Gonzalez de Villambrosia S, Rodriguez A, et al. Primary cutaneous anaplastic large cell lymphomas with 6p25.3 rearrangement exhibit particular histological features. Histopathology. 2015;66:846-55 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Takahashi Y, Takata K, Kato S, Sato Y, Asano N, Ogino T, et al. Clinicopathological analysis of 17 primary cutaneous T-cell lymphoma of the ?? phenotype from Japan. Cancer Sci. 2014;105:912-23 pubmed publisher
  • immunohistochemistry - paraffin section; human; 3 ug/ml; loading ...; fig 4
Helm O, Mennrich R, Petrick D, Goebel L, Freitag Wolf S, Roder C, et al. Comparative characterization of stroma cells and ductal epithelium in chronic pancreatitis and pancreatic ductal adenocarcinoma. PLoS ONE. 2014;9:e94357 pubmed publisher
  • immunohistochemistry; human; 1:50
Kempf W, Kazakov D, Broekaert S, Metze D. Pediatric CD8?CD56? non-poikilodermatous mycosis fungoides: case report and review of the literature. Am J Dermatopathol. 2014;36:598-602 pubmed publisher
  • immunoprecipitation; human
Behnke J, Hendershot L. The large Hsp70 Grp170 binds to unfolded protein substrates in vivo with a regulation distinct from conventional Hsp70s. J Biol Chem. 2014;289:2899-907 pubmed publisher
  • immunohistochemistry; human; 1:50
Kempf W, Kazakov D, Paredes B, Laeng H, Palmedo G, Kutzner H. Primary cutaneous anaplastic large cell lymphoma with angioinvasive features and cytotoxic phenotype: a rare lymphoma variant within the spectrum of CD30+ lymphoproliferative disorders. Dermatology. 2013;227:346-52 pubmed publisher
  • immunohistochemistry; human; 1:50
Hayashi E, Takata K, Sato Y, Tashiro Y, Tachiyama Y, Sawada Kitamura S, et al. Distinct morphologic, phenotypic, and clinical-course characteristics of indolent peripheral T-cell lymphoma. Hum Pathol. 2013;44:1927-36 pubmed publisher
  • immunohistochemistry; human; 1:40
Wang T, Xu C, Liu S, Kan B, Ran Y, Liu W, et al. Clinicopathology, immunophenotype, T cell receptor gene rearrangement, Epstein-Barr virus status and p53 gene mutation of cutaneous extranodal NK/T-cell lymphoma, nasal-type. Chin Med J (Engl). 2013;126:1281-7 pubmed
  • immunohistochemistry; human; 1:25
Wilson A, Swerdlow S, Przybylski G, Surti U, Choi J, Campo E, et al. Intestinal ?? T-cell lymphomas are most frequently of type II enteropathy-associated T-cell type. Hum Pathol. 2013;44:1131-45 pubmed publisher
  • immunohistochemistry; human; 1:40
Ogura R, Aoki H, Natsumeda M, Shimizu H, Kobayashi T, Saito T, et al. Epstein-Barr virus-associated primary central nervous system cytotoxic T-cell lymphoma. Neuropathology. 2013;33:436-41 pubmed publisher
  • immunohistochemistry; human
Ishida M, Mochizuki Y, Saito Y, Fujiyama Y, Hodohara K, Yoshida T, et al. CD8(+) mycosis fungoides with esophageal involvement: A case report. Oncol Lett. 2013;5:73-75 pubmed
  • immunohistochemistry; human; 1:50
Kempf W, Kazakov D, Schärer L, Rutten A, Mentzel T, Paredes B, et al. Angioinvasive lymphomatoid papulosis: a new variant simulating aggressive lymphomas. Am J Surg Pathol. 2013;37:1-13 pubmed publisher
  • flow cytometry; human
  • immunohistochemistry; human; 1:100
  • western blot; human
Beham A, Puellmann K, Laird R, Fuchs T, Streich R, Breysach C, et al. A TNF-regulated recombinatorial macrophage immune receptor implicated in granuloma formation in tuberculosis. PLoS Pathog. 2011;7:e1002375 pubmed publisher
  • ELISA; human; 1:1500
Leimgruber A, Ferber M, Irving M, Hussain Kahn H, Wieckowski S, Derre L, et al. TCRep 3D: an automated in silico approach to study the structural properties of TCR repertoires. PLoS ONE. 2011;6:e26301 pubmed publisher
  • immunohistochemistry; human; fig 3
Lee D, Yang J, Lee S, Won C, Chang S, Lee M, et al. Subcutaneous panniculitis-like T-cell lymphoma: a clinical and pathologic study of 14 korean patients. Ann Dermatol. 2011;23:329-37 pubmed publisher
  • immunohistochemistry; human
Chen Y, Chadburn A, Evens A, Winter J, Gordon L, Chenn A, et al. Clinical, morphologic, immunophenotypic, and molecular cytogenetic assessment of CD4-/CD8-?? T-cell large granular lymphocytic leukemia. Am J Clin Pathol. 2011;136:289-99 pubmed publisher
  • immunohistochemistry; human
Garcia Herrera A, Song J, Chuang S, Villamor N, Colomo L, Pittaluga S, et al. Nonhepatosplenic γδ T-cell lymphomas represent a spectrum of aggressive cytotoxic T-cell lymphomas with a mainly extranodal presentation. Am J Surg Pathol. 2011;35:1214-25 pubmed publisher
  • immunohistochemistry; human
Hocker T, Wada D, McPhail E, Porrata L, El Azhary R, Gibson L. Relapsed hepatosplenic T-cell lymphoma heralded by a solitary skin nodule. J Cutan Pathol. 2011;38:899-904 pubmed publisher
  • immunohistochemistry; human
Lu C, Tang Y, Yang Q, Wang M, Zhao S, Bi C, et al. Hepatosplenic T-cell lymphoma: clinicopathologic, immunophenotypic, and molecular characterization of 17 Chinese cases. Hum Pathol. 2011;42:1965-78 pubmed publisher
  • immunohistochemistry; mouse
Sun J, Lu Z, Yang D, Chen J. Primary intestinal T-cell and NK-cell lymphomas: a clinicopathological and molecular study from China focused on type II enteropathy-associated T-cell lymphoma and primary intestinal NK-cell lymphoma. Mod Pathol. 2011;24:983-92 pubmed publisher
  • immunohistochemistry; human
Takeshita M, Nakamura S, Kikuma K, Nakayama Y, Nimura S, Yao T, et al. Pathological and immunohistological findings and genetic aberrations of intestinal enteropathy-associated T cell lymphoma in Japan. Histopathology. 2011;58:395-407 pubmed publisher
  • flow cytometry; fission yeast
Aggen D, Chervin A, Insaidoo F, Piepenbrink K, Baker B, Kranz D. Identification and engineering of human variable regions that allow expression of stable single-chain T cell receptors. Protein Eng Des Sel. 2011;24:361-72 pubmed publisher
  • immunohistochemistry; human; 1:40
Geissinger E, Sadler P, Roth S, Grieb T, Puppe B, Müller N, et al. Disturbed expression of the T-cell receptor/CD3 complex and associated signaling molecules in CD30+ T-cell lymphoproliferations. Haematologica. 2010;95:1697-704 pubmed publisher
  • immunohistochemistry; human
Berti E, Recalcati S, Girgenti V, Fanoni D, Venegoni L, Vezzoli P. Cutaneous extranodal NK/T-cell lymphoma: a clinicopathologic study of 5 patients with array-based comparative genomic hybridization. Blood. 2010;116:165-70 pubmed publisher
  • immunohistochemistry; human; 1:100
Feldman A, Law M, Inwards D, Dogan A, McClure R, Macon W. PAX5-positive T-cell anaplastic large cell lymphomas associated with extra copies of the PAX5 gene locus. Mod Pathol. 2010;23:593-602 pubmed publisher
  • immunohistochemistry; human
Kawamoto K, Nakamura S, Iwashita A, Watanabe J, Oshiro Y, Nakayama Y, et al. Clinicopathological characteristics of primary gastric T-cell lymphoma. Histopathology. 2009;55:641-53 pubmed publisher
  • immunohistochemistry; human
Chang S, Liao Y, Lin S, Chuang S. NK-cell lymphoma with nodal presentation and expression of cutaneous lymphocyte-associated antigen. Pathol Res Pract. 2010;206:463-6 pubmed publisher
  • immunohistochemistry; human; 1:80
Roullet M, Gheith S, Mauger J, Junkins Hopkins J, Choi J. Percentage of {gamma}{delta} T cells in panniculitis by paraffin immunohistochemical analysis. Am J Clin Pathol. 2009;131:820-6 pubmed publisher
  • flow cytometry; human
RICHMAN S, Aggen D, Dossett M, Donermeyer D, Allen P, Greenberg P, et al. Structural features of T cell receptor variable regions that enhance domain stability and enable expression as single-chain ValphaVbeta fragments. Mol Immunol. 2009;46:902-16 pubmed publisher
  • immunohistochemistry; human
Kong Y, Dai B, Kong J, Zhou X, Lu H, Shen L, et al. Subcutaneous panniculitis-like T-cell lymphoma: a clinicopathologic, immunophenotypic, and molecular study of 22 Asian cases according to WHO-EORTC classification. Am J Surg Pathol. 2008;32:1495-502 pubmed publisher
  • flow cytometry; human
Rizzo K, Stetler Stevenson M, Wilson W, Yuan C. Novel CD19 expression in a peripheral T cell lymphoma: A flow cytometry case report with morphologic correlation. Cytometry B Clin Cytom. 2009;76:142-9 pubmed publisher
  • immunohistochemistry; human
Grogg K, Jung S, Erickson L, McClure R, Dogan A. Primary cutaneous CD4-positive small/medium-sized pleomorphic T-cell lymphoma: a clonal T-cell lymphoproliferative disorder with indolent behavior. Mod Pathol. 2008;21:708-15 pubmed publisher
  • immunohistochemistry; human
  • western blot; human
Puellmann K, Kaminski W, Vogel M, Nebe C, Schroeder J, Wolf H, et al. A variable immunoreceptor in a subpopulation of human neutrophils. Proc Natl Acad Sci U S A. 2006;103:14441-6 pubmed
  • immunohistochemistry; human; 1:80
Wu H, Said J, Ames E, Chen C, McWhorter V, Chen P, et al. First reported cases of intravascular large cell lymphoma of the NK cell type: clinical, histologic, immunophenotypic, and molecular features. Am J Clin Pathol. 2005;123:603-11 pubmed
Liu C, Chen B, Chuang S. Malignant effusions from extranodal NK/T-cell lymphomas are frequently of anaplastic morphology with azurophilic granules and of T-cell lineage. Diagn Cytopathol. 2020;48:453-463 pubmed publisher
Jungbluth A, Frosina D, Fayad M, Pulitzer M, Dogan A, Busam K, et al. Immunohistochemical Detection of γ/δ T Lymphocytes in Formalin-fixed Paraffin-embedded Tissues. Appl Immunohistochem Mol Morphol. 2019;27:581-583 pubmed publisher
Ali L, Young M, Bayerl M, Helm K, Clarke L. Gamma-delta T-cell lymphoma arising in a long-standing cutaneous plaque. J Cutan Pathol. 2015;42:987-991 pubmed publisher
Feige M, Hendershot L. Quality control of integral membrane proteins by assembly-dependent membrane integration. Mol Cell. 2013;51:297-309 pubmed publisher
Irving M, Zoete V, Hebeisen M, Schmid D, Baumgärtner P, Guillaume P, et al. Interplay between T cell receptor binding kinetics and the level of cognate peptide presented by major histocompatibility complexes governs CD8+ T cell responsiveness. J Biol Chem. 2012;287:23068-78 pubmed publisher
Streubel B, Chott A, Huber D, Exner M, Jager U, Wagner O, et al. Lymphoma-specific genetic aberrations in microvascular endothelial cells in B-cell lymphomas. N Engl J Med. 2004;351:250-9 pubmed
Taskinen M, Westerholm Ormio M, Karikoski R, Lindahl H, Veres G, Savilahti E, et al. Increased cell turnover, but no signs of increased T-cell infiltration or inflammatory cytokines in the duodenum of pediatric patients after allogeneic stem cell transplantation. Bone Marrow Transplant. 2004;34:221-8 pubmed
Takeshita M, Imayama S, Oshiro Y, Kurihara K, Okamoto S, Matsuki Y, et al. Clinicopathologic analysis of 22 cases of subcutaneous panniculitis-like CD56- or CD56+ lymphoma and review of 44 other reported cases. Am J Clin Pathol. 2004;121:408-16 pubmed
Westerholm Ormio M, Vaarala O, Pihkala P, Ilonen J, Savilahti E. Immunologic activity in the small intestinal mucosa of pediatric patients with type 1 diabetes. Diabetes. 2003;52:2287-95 pubmed
Torres P, Alcover A, Zapata D, Arnaud J, Pacheco A, Martín Fernández J, et al. TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J Immunol. 2003;170:5947-55 pubmed
Westerholm Ormio M, Garioch J, Ketola I, Savilahti E. Inflammatory cytokines in small intestinal mucosa of patients with potential coeliac disease. Clin Exp Immunol. 2002;128:94-101 pubmed
Taghon T, De Smedt M, Stolz F, Cnockaert M, Plum J, Leclercq G. Enforced expression of GATA-3 severely reduces human thymic cellularity. J Immunol. 2001;167:4468-75 pubmed
Hyjek E, Chadburn A, Liu Y, Cesarman E, Knowles D. BCL-6 protein is expressed in precursor T-cell lymphoblastic lymphoma and in prenatal and postnatal thymus. Blood. 2001;97:270-6 pubmed
Katoh A, Ohshima K, Kanda M, Haraoka S, Sugihara M, Suzumiya J, et al. Gastrointestinal T cell lymphoma: predominant cytotoxic phenotypes, including alpha/beta, gamma/delta T cell and natural killer cells. Leuk Lymphoma. 2000;39:97-111 pubmed
Babbe H, Roers A, Waisman A, Lassmann H, Goebels N, Hohlfeld R, et al. Clonal expansions of CD8(+) T cells dominate the T cell infiltrate in active multiple sclerosis lesions as shown by micromanipulation and single cell polymerase chain reaction. J Exp Med. 2000;192:393-404 pubmed
Appel H, Gauthier L, Pyrdol J, Wucherpfennig K. Kinetics of T-cell receptor binding by bivalent HLA-DR. Peptide complexes that activate antigen-specific human T-cells. J Biol Chem. 2000;275:312-21 pubmed
Wu H, Zhang G, Clarkson A, Knight J. Conserved T-cell receptor beta chain CDR3 sequences in IgA nephropathy biopsies. Kidney Int. 1999;55:109-19 pubmed
Sano S, Matsui Y, Itami S, Yoshikawa K. Immunological study on CD3 defective cutaneous T cell lymphoma cells from a patient with Sézary syndrome. Clin Exp Immunol. 1998;113:190-7 pubmed
Calaman S, Carson G, Henry L, Kubinec J, Kuestner R, Ahmed A, et al. Characterization of monoclonal antibodies specific for the V beta 3 family of the human T cell receptor generated using soluble TCR beta-chain. J Immunol Methods. 1993;164:233-44 pubmed
Lohman B, Miller C, McChesney M. Antiviral cytotoxic T lymphocytes in vaginal mucosa of simian immunodeficiency virus-infected rhesus macaques. J Immunol. 1995;155:5855-60 pubmed
Campana D, Janossy G, Coustan Smith E, Amlot P, Tian W, Ip S, et al. The expression of T cell receptor-associated proteins during T cell ontogeny in man. J Immunol. 1989;142:57-66 pubmed
Falini B, Flenghi L, Pileri S, Pelicci P, Fagioli M, Martelli M, et al. Distribution of T cells bearing different forms of the T cell receptor gamma/delta in normal and pathological human tissues. J Immunol. 1989;143:2480-8 pubmed
Rink M. Rapid identification of organisms from blood cultures. Med Lab Sci. 1990;47:6-9 pubmed
Jacobs M, Haynes B. Increase in TCR gamma delta T lymphocytes in synovia from rheumatoid arthritis patients with active synovitis. J Clin Immunol. 1992;12:130-8 pubmed
product information
Product Type :
Antibody
Product Name :
TCR V beta F1 Monoclonal Antibody (8A3)
Catalog # :
TCR1151
Quantity :
200 ug
Price :
US 491.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 2 ug/test, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent
Species :
Human
Clone :
8A3
Isotype :
IgG1
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
The T Cell Receptor alpha beta (TCRab) is a heterodimeric membrane receptor that consists of the highly variable alpha and beta chains expressed as part of a complex with the invariant CD3 proteins. TCRab participates in the activation of T-cells in response to an antigen presented in the context of MHC. Engagement of the TCRab initiates signaling cascades that ultimately result in cellular proliferation, differentiation, cytokine production, and/or activation-induced cell death. These signaling cascades regulate T-cell development, homeostasis, activation, acquisition of effector functions and apoptosis. Cells expressing TCRab constitute the vast majority of T cells in both human and mouse.
Immunogen :
Human TCR chain constant region
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 2 ug/test, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent
Aliases :
T cell receptor beta locus; tcr beta f1; TCRB; TRB; TRB@
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA