CD11b Monoclonal Antibody (M1/70.15), PE | Invitrogen RM2804-3 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD11b Monoclonal Antibody (M1/70.15), PE

catalog :

RM2804-3

quantity :

3 mL

price :

524 USD

clonality :

monoclonal

host :

rat

conjugate :

clone name :

M1/70.15

reactivity :

human, mouse

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:200; fig 4	Stasulli N, Eichelberger K, Price P, Pechous R, Montgomery S, Parker J, et al. Spatially distinct neutrophil responses within the inflammatory lesions of pneumonic plague. MBio. 2015;6:e01530-15 pubmed publisher
flow cytometry; mouse; 1:500; fig 4	Pechous R, Broberg C, Stasulli N, Miller V, Goldman W. In vivo transcriptional profiling of Yersinia pestis reveals a novel bacterial mediator of pulmonary inflammation. MBio. 2015;6:e02302-14 pubmed publisher
flow cytometry; mouse	Sudres M, Norol F, Trenado A, Gregoire S, Charlotte F, Levacher B, et al. Bone marrow mesenchymal stem cells suppress lymphocyte proliferation in vitro but fail to prevent graft-versus-host disease in mice. J Immunol. 2006;176:7761-7 pubmed
flow cytometry; mouse; fig 4	Grisaru D, Pick M, Perry C, Sklan E, Almog R, Goldberg I, et al. Hydrolytic and nonenzymatic functions of acetylcholinesterase comodulate hemopoietic stress responses. J Immunol. 2006;176:27-35 pubmed
flow cytometry; mouse	Morin J, Faideau B, Gagnerault M, Lepault F, Boitard C, Boudaly S. Passive transfer of flt-3L-derived dendritic cells delays diabetes development in NOD mice and associates with early production of interleukin (IL)-4 and IL-10 in the spleen of recipient mice. Clin Exp Immunol. 2003;134:388-95 pubmed
flow cytometry; mouse	Melani C, Chiodoni C, Forni G, Colombo M. Myeloid cell expansion elicited by the progression of spontaneous mammary carcinomas in c-erbB-2 transgenic BALB/c mice suppresses immune reactivity. Blood. 2003;102:2138-45 pubmed
flow cytometry; mouse	Gilbert K, Boger S, Fifer E. Butyric acid derivative induces allospecific T cell anergy and prevents graft-versus-host disease. Immunopharmacol Immunotoxicol. 2003;25:13-27 pubmed
flow cytometry; mouse	Masuya M, Drake C, Fleming P, Reilly C, Zeng H, Hill W, et al. Hematopoietic origin of glomerular mesangial cells. Blood. 2003;101:2215-8 pubmed
flow cytometry; mouse	Carter R, Campbell I, O Donnel K, Wicks I. Vascular cell adhesion molecule-1 (VCAM-1) blockade in collagen-induced arthritis reduces joint involvement and alters B cell trafficking. Clin Exp Immunol. 2002;128:44-51 pubmed
flow cytometry; mouse; fig 5	Sonoda K, Taniguchi M, Stein Streilein J. Long-term survival of corneal allografts is dependent on intact CD1d-reactive NKT cells. J Immunol. 2002;168:2028-34 pubmed
flow cytometry; mouse; fig 5	Lehmann J, Bellmann S, Werner C, Schroder R, Schutze N, Alber G. IL-12p40-dependent agonistic effects on the development of protective innate and adaptive immunity against Salmonella enteritidis. J Immunol. 2001;167:5304-15 pubmed
flow cytometry; mouse	Kawada H, Ogawa M. Bone marrow origin of hematopoietic progenitors and stem cells in murine muscle. Blood. 2001;98:2008-13 pubmed
flow cytometry; mouse	Campbell I, O DONNELL K, Lawlor K, Wicks I. Severe inflammatory arthritis and lymphadenopathy in the absence of TNF. J Clin Invest. 2001;107:1519-27 pubmed
flow cytometry; mouse	Vuola J, Puurula V, Anttila M, Makela P, Rautonen N. Acquired immunity to Chlamydia pneumoniae is dependent on gamma interferon in two mouse strains that initially differ in this respect after primary challenge. Infect Immun. 2000;68:960-4 pubmed
flow cytometry; mouse; fig 1	Lepault F, Gagnerault M. Characterization of peripheral regulatory CD4+ T cells that prevent diabetes onset in nonobese diabetic mice. J Immunol. 2000;164:240-7 pubmed
flow cytometry; mouse	Dejbakhsh Jones S, Strober S. Identification of an early T cell progenitor for a pathway of T cell maturation in the bone marrow. Proc Natl Acad Sci U S A. 1999;96:14493-8 pubmed
flow cytometry; mouse	Sonoda K, Exley M, Snapper S, Balk S, Stein Streilein J. CD1-reactive natural killer T cells are required for development of systemic tolerance through an immune-privileged site. J Exp Med. 1999;190:1215-26 pubmed
flow cytometry; mouse	Batteux F, Tourneur L, Trebeden H, Charreire J, Chiocchia G. Gene therapy of experimental autoimmune thyroiditis by in vivo administration of plasmid DNA coding for Fas ligand. J Immunol. 1999;162:603-8 pubmed
flow cytometry; mouse; fig 3, 4	Moritz D, Rodewald H, Gheyselinck J, Klemenz R. The IL-1 receptor-related T1 antigen is expressed on immature and mature mast cells and on fetal blood mast cell progenitors. J Immunol. 1998;161:4866-74 pubmed
flow cytometry; mouse	Pear W, Aster J, Scott M, Hasserjian R, Soffer B, Sklar J, et al. Exclusive development of T cell neoplasms in mice transplanted with bone marrow expressing activated Notch alleles. J Exp Med. 1996;183:2283-91 pubmed
	Reed M, Luissint A, Azcutia V, Fan S, O Leary M, Quirós M, et al. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo. Nat Commun. 2019;10:5004 pubmed publisher
	Hsieh C, Niemi E, Wang S, Lee C, Bingham D, Zhang J, et al. CCR2 deficiency impairs macrophage infiltration and improves cognitive function after traumatic brain injury. J Neurotrauma. 2014;31:1677-88 pubmed publisher
	Carlow D, Gold M, Ziltener H. Lymphocytes in the peritoneum home to the omentum and are activated by resident dendritic cells. J Immunol. 2009;183:1155-65 pubmed publisher
	Amano S, Sekine K, Bonewald L, Ohmori Y. A novel osteoclast precursor cell line, 4B12, recapitulates the features of primary osteoclast differentiation and function: enhanced transfection efficiency before and after differentiation. J Cell Physiol. 2009;221:40-53 pubmed publisher
	Nakao S, Hata Y, Miura M, Noda K, Kimura Y, Kawahara S, et al. Dexamethasone inhibits interleukin-1beta-induced corneal neovascularization: role of nuclear factor-kappaB-activated stromal cells in inflammatory angiogenesis. Am J Pathol. 2007;171:1058-65 pubmed
	Nakao S, Kuwano T, Tsutsumi Miyahara C, Ueda S, Kimura Y, Hamano S, et al. Infiltration of COX-2-expressing macrophages is a prerequisite for IL-1 beta-induced neovascularization and tumor growth. J Clin Invest. 2005;115:2979-91 pubmed
	Kabashima K, Banks T, Ansel K, Lu T, Ware C, Cyster J. Intrinsic lymphotoxin-beta receptor requirement for homeostasis of lymphoid tissue dendritic cells. Immunity. 2005;22:439-50 pubmed
	Watts G, Beurskens F, Martin Padura I, Ballantyne C, Klickstein L, Brenner M, et al. Manifestations of inflammatory arthritis are critically dependent on LFA-1. J Immunol. 2005;174:3668-75 pubmed

product information

Product Type :

Antibody

Product Name :

CD11b Monoclonal Antibody (M1/70.15), PE

Catalog # :

RM2804-3

Quantity :

3 mL

Price :

524 USD

Clonality :

Monoclonal

Purity :

purified

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: Assay Dependent

Species :

Mouse

Clone :

M1/70.15

Isotype :

IgG2b

Storage :

4° C

Description :

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

Immunogen :

C57BL/10 splenic T cells and concanavalin A stimulated C57BL/10 splenocytes

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay Dependent

Aliases :

antigen CD11b (p170); antigen CD11b (p170); macrophage antigen alpha polypeptide; CD11 antigen-like family member B; CD11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha M; integrin alpha-M; integrin subunit alpha M; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); Itgam; Leukocyte adhesion receptor MO1; leukocyte integrin alpha-M chain; Ly-40; MAC1; Mac-1; Mac-1 alpha; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6; unnamed protein product

more info or order :

Invitrogen product webpage