IL-10 Monoclonal Antibody (JES3-9D7), PE | Invitrogen RHCIL1004 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

IL-10 Monoclonal Antibody (JES3-9D7), PE

catalog :

RHCIL1004

quantity :

500µL

price :

164 USD

clonality :

monoclonal

host :

rat

conjugate :

clone name :

JES3-9D7

reactivity :

human, Rhesus monkey

application :

flow cytometry, immunohistochemistry - frozen section

citations: 13

Published Application/Species/Sample/Dilution	Reference
ELISA; human; loading ...; fig 5c	Otsuka Y, Watanabe E, Shinya E, Okura S, Saeki H, Geijtenbeek T, et al. Differentiation of Langerhans Cells from Monocytes and Their Specific Function in Inducing IL-22-Specific Th Cells. J Immunol. 2018;201:3006-3016 pubmed publisher
flow cytometry; human; fig 4	He Y, He X, Guo P, Du M, Shao J, Li M, et al. The decidual stromal cells-secreted CCL2 induces and maintains decidual leukocytes into Th2 bias in human early pregnancy. Clin Immunol. 2012;145:161-73 pubmed publisher
flow cytometry; Rhesus monkey	Souza Lemos C, de Campos S, Teva A, Porrozzi R, Grimaldi G. In situ characterization of the granulomatous immune response with time in nonhealing lesional skin of Leishmania braziliensis-infected rhesus macaques (Macaca mulatta). Vet Immunol Immunopathol. 2011;142:147-55 pubmed publisher
immunohistochemistry - frozen section; human; 1:20; fig 1	Syrio N, Faria D, Gomez R, Gollob K, Dutra W, Souza P. IL-10 and IL-10 receptor overexpression in oral giant cell lesions. Med Oral Patol Oral Cir Bucal. 2011;16:e488-92 pubmed
flow cytometry; human; tbl 1	Laso F, Almeida J, Torres E, Vaquero J, Marcos M, Orfao A. Chronic alcohol consumption is associated with an increased cytotoxic profile of circulating lymphocytes that may be related with the development of liver injury. Alcohol Clin Exp Res. 2010;34:876-85 pubmed publisher
flow cytometry; human; fig 3	Della Bella S, Giannelli S, Taddeo A, Presicce P, Villa M. Application of six-color flow cytometry for the assessment of dendritic cell responses in whole blood assays. J Immunol Methods. 2008;339:153-64 pubmed publisher
flow cytometry; human; fig 2	Kirchberger S, Majdic O, Steinberger P, Bluml S, Pfistershammer K, Zlabinger G, et al. Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression. J Immunol. 2005;175:1145-52 pubmed
flow cytometry; Rhesus monkey; fig 4, 5	Gansuvd B, Hubbard W, Hutchings A, Thomas F, Goodwin J, Wilson S, et al. Phenotypic and functional characterization of long-term cultured rhesus macaque spleen-derived NKT cells. J Immunol. 2003;171:2904-11 pubmed
flow cytometry; human; fig 1	Metelitsa L, Naidenko O, Kant A, Wu H, Loza M, Perussia B, et al. Human NKT cells mediate antitumor cytotoxicity directly by recognizing target cell CD1d with bound ligand or indirectly by producing IL-2 to activate NK cells. J Immunol. 2001;167:3114-22 pubmed
flow cytometry; human	Van Damme N, De Keyser F, Demetter P, Baeten D, Mielants H, Verbruggen G, et al. The proportion of Th1 cells, which prevail in gut mucosa, is decreased in inflammatory bowel syndrome. Clin Exp Immunol. 2001;125:383-90 pubmed
flow cytometry; human	Koshiba M, Rosin D, Hayashi N, Linden J, Sitkovsky M. Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies. Mol Pharmacol. 1999;55:614-24 pubmed
ELISA; human	Salgame P, Abrams J, Clayberger C, Goldstein H, Convit J, Modlin R, et al. Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones. Science. 1991;254:279-82 pubmed
ELISA; human; fig 1	Yssel H, de Waal Malefyt R, Roncarolo M, Abrams J, Lahesmaa R, Spits H, et al. IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells. J Immunol. 1992;149:2378-84 pubmed

product information

Product Type :

Antibody

Product Name :

IL-10 Monoclonal Antibody (JES3-9D7), PE

Catalog # :

RHCIL1004

Quantity :

500µL

Price :

164 USD

Clonality :

Monoclonal

Purity :

purified

Host :

Rat

Reactivity :

Human

Applications :

Flow Cytometry: 5 µL / 1x10^6 cells

Species :

Human

Clone :

JES3-9D7

Isotype :

IgG1, kappa

Storage :

4° C

Description :

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

Immunogen :

Recombinant human Interleukin 10.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 5 µL / 1x10^6 cells

Aliases :

CSIF, IL10A, MGC126450, MGC126451, TGIF, Interleukin-10