This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
HisG Epitope Tag Monoclonal Antibody, AP
catalog :
R942-25
quantity :
125 uL
price :
US 412.00
clonality :
monoclonal
host :
mouse
conjugate :
AP
application :
western blot
product information
Product Type :
Antibody
Product Name :
HisG Epitope Tag Monoclonal Antibody, AP
Catalog # :
R942-25
Quantity :
125 uL
Price :
US 412.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Tag
Applications :
Western Blot: 1:2,000
Species :
Tag
Isotype :
IgG2a
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. HisG antibodies allow detection of recombinant proteins containing a polyhistidine sequence: His-His-His-His-His-His-Gly (6xHis-Gly epitope). HisG antibodies can be used to detect expression of fusion proteins from bacterial, insect, and mammalian cells.
Immunogen :
6x His synthetic peptide: -His-His-His-His-His-His-Gly
Format :
Liquid
Applications w/Dilutions :
Western Blot: 1:2,000
Aliases :
6xHis-Gly; 6xHis-Glycine; His G; His Tag; Histidine Tag
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA