HisG Epitope Tag Monoclonal Antibody, HRP | Invitrogen R941-25 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HisG Epitope Tag Monoclonal Antibody, HRP

catalog :

R941-25

quantity :

50 uL

price :

US 404.00

clonality :

monoclonal

host :

mouse

conjugate :

HRP

application :

western blot, ELISA

citations: 4

Reference
Yoo S, Lee C, An H, Lee J, Lee H, Kang H, et al. RSK2-Mediated ELK3 Activation Enhances Cell Transformation and Breast Cancer Cell Growth by Regulation of c-fos Promoter Activity. Int J Mol Sci. 2019;20: pubmed publisher
Parikh R, Lin H, Gangaraju V. A critical role for nucleoporin 358 (Nup358) in transposon silencing and piRNA biogenesis in Drosophila. J Biol Chem. 2018;293:9140-9147 pubmed publisher
Maharaj K, Que N, Hong F, Huck J, Gill S, Wu S, et al. Exploring the Functional Complementation between Grp94 and Hsp90. PLoS ONE. 2016;11:e0166271 pubmed publisher
Plaisier C, Horvath S, Huertas Vazquez A, Cruz Bautista I, Herrera M, Tusie Luna T, et al. A systems genetics approach implicates USF1, FADS3, and other causal candidate genes for familial combined hyperlipidemia. PLoS Genet. 2009;5:e1000642 pubmed publisher

product information

Product Type :

Antibody

Product Name :

HisG Epitope Tag Monoclonal Antibody, HRP

Catalog # :

R941-25

Quantity :

50 uL

Price :

US 404.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Tag

Applications :

ELISA: Assay-dependent, Western Blot: 1:1,000

Species :

Tag

Isotype :

IgG2a

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. HisG antibodies allow detection of recombinant proteins containing a polyhistidine sequence: His-His-His-His-His-His-Gly (6xHis-Gly epitope). HisG antibodies can be used to detect expression of fusion proteins from bacterial, insect, and mammalian cells.

Immunogen :

6x His synthetic peptide: -His-His-His-His-His-His-Gly

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Western Blot: 1:1,000

Aliases :

6xHis-Gly; 6xHis-Glycine; His G; His Tag; Histidine Tag