HisG Epitope Tag Monoclonal Antibody | Invitrogen R940-25 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HisG Epitope Tag Monoclonal Antibody

catalog :

R940-25

quantity :

50 uL

price :

US 341.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, other

citations: 16

Reference
Bieluszewski T, Sura W, Dziegielewski W, Bieluszewska A, Lachance C, Kabza M, et al. NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis. Nat Commun. 2022;13:277 pubmed publisher
Bieluszewska A, Weglewska M, Bieluszewski T, Lesniewicz K, Poreba E. PKA-binding domain of AKAP8 is essential for direct interaction with DPY30 protein. FEBS J. 2018;285:947-964 pubmed publisher
Hauck L, Grothe D, Billia F. p21(CIP1/WAF1)-dependent inhibition of cardiac hypertrophy in response to Angiotensin II involves Akt/Myc and pRb signaling. Peptides. 2016;83:38-48 pubmed publisher
Braley A, Kwak T, Jules J, Harja E, Landgraf R, Hudson B. Regulation of Receptor for Advanced Glycation End Products (RAGE) Ectodomain Shedding and Its Role in Cell Function. J Biol Chem. 2016;291:12057-73 pubmed publisher
Gram A, Fox B, BÃ¼chler U, Boos A, Hoffmann B, Kowalewski M. Canine placental prostaglandin E2 synthase: expression, localization, and biological functions in providing substrates for prepartum PGF2alpha synthesis. Biol Reprod. 2014;91:154 pubmed publisher
Mu N, Liu N, Hao Q, Xu Y, Li J, Li W, et al. Inhibition of mouse SP2/0 myeloma cell growth by the B7-H4 protein vaccine. BMB Rep. 2014;47:399-404 pubmed
Gram A, B chler U, Boos A, Hoffmann B, Kowalewski M. Biosynthesis and degradation of canine placental prostaglandins: prepartum changes in expression and function of prostaglandin F2?-synthase (PGFS, AKR1C3) and 15-hydroxyprostaglandin dehydrogenase (HPGD). Biol Reprod. 2013;89:2 pubmed publisher
Chen K, Chang C, Huang C, Lin C, Lin W, Lo Y, et al. TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity. J Biol Chem. 2012;287:32216-21 pubmed publisher
Wang S, Nan K, Wang Y, Wang W, Tian T. The balance between two isoforms of LEF-1 regulates colon carcinoma growth. BMC Gastroenterol. 2012;12:53 pubmed publisher
Galetto L, Bosco D, Balestrini R, Genre A, Fletcher J, Marzachì C. The major antigenic membrane protein of "Candidatus Phytoplasma asteris" selectively interacts with ATP synthase and actin of leafhopper vectors. PLoS ONE. 2011;6:e22571 pubmed publisher
Hu Y, Janitz M. High-throughput subcellular protein localization using transfected-cell arrays. Subcellular protein localization using cell arrays. Methods Mol Biol. 2011;706:53-72 pubmed publisher
Ghanekar A, Mendicino M, Liu H, He W, Liu M, Zhong R, et al. Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection. J Immunol. 2004;172:5693-701 pubmed
Meng W, Numazaki M, Takeuchi K, Uchibori Y, Ando Akatsuka Y, Tominaga M, et al. DIP (mDia interacting protein) is a key molecule regulating Rho and Rac in a Src-dependent manner. EMBO J. 2004;23:760-71 pubmed
Liu B, Peng D, Lu Y, Jin W, Fan Z. A novel single amino acid deletion caspase-8 mutant in cancer cells that lost proapoptotic activity. J Biol Chem. 2002;277:30159-64 pubmed
Hirohata S, Wang L, Miyagi M, Yan L, Seldin M, Keene D, et al. Punctin, a novel ADAMTS-like molecule, ADAMTSL-1, in extracellular matrix. J Biol Chem. 2002;277:12182-9 pubmed
Sommer K, Chen L, Treuting P, Smith L, Swisshelm K. Elevated retinoic acid receptor beta(4) protein in human breast tumor cells with nuclear and cytoplasmic localization. Proc Natl Acad Sci U S A. 1999;96:8651-6 pubmed

product information

Product Type :

Antibody

Product Name :

HisG Epitope Tag Monoclonal Antibody

Catalog # :

R940-25

Quantity :

50 uL

Price :

US 341.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Tag

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 1:250, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:2,000

Species :

Tag

Isotype :

IgG2a

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. HisG antibodies allow detection of recombinant proteins containing a polyhistidine sequence: His-His-His-His-His-His-Gly (6xHis-Gly epitope). HisG antibodies can be used to detect expression of fusion proteins from bacterial, insect, and mammalian cells.

Immunogen :

6x His synthetic peptide: -His-His-His-His-His-His-Gly

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 1:250, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:2,000

Aliases :

6xHis-Gly; 6xHis-Glycine; His G; His Tag; Histidine Tag