6x-His Tag (C-term) Monoclonal Antibody (3D5), FITC | Invitrogen R933-25 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

6x-His Tag (C-term) Monoclonal Antibody (3D5), FITC

catalog :

R933-25

quantity :

50 uL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

3D5

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 5

Reference
Peng Y, Miao H, Wu S, Yang W, Zhang Y, Xie G, et al. ABHD5 interacts with BECN1 to regulate autophagy and tumorigenesis of colon cancer independent of PNPLA2. Autophagy. 2016;12:2167-2182 pubmed
Salinas N, Tolia N. A quantitative assay for binding and inhibition of Plasmodium falciparum Erythrocyte Binding Antigen 175 reveals high affinity binding depends on both DBL domains. Protein Expr Purif. 2014;95:188-94 pubmed publisher
Verhelst K, Carpentier I, Kreike M, Meloni L, Verstrepen L, Kensche T, et al. A20 inhibits LUBAC-mediated NF-?B activation by binding linear polyubiquitin chains via its zinc finger 7. EMBO J. 2012;31:3845-55 pubmed publisher
Brennan F, Green P, Amjadi P, Robertshaw H, Alvarez Iglesias M, Takata M. Interleukin-10 regulates TNF-alpha-converting enzyme (TACE/ADAM-17) involving a TIMP-3 dependent and independent mechanism. Eur J Immunol. 2008;38:1106-17 pubmed publisher
Hassouni M, Chambost J, Expert D, Van Gijsegem F, Barras F. The minimal gene set member msrA, encoding peptide methionine sulfoxide reductase, is a virulence determinant of the plant pathogen Erwinia chrysanthemi. Proc Natl Acad Sci U S A. 1999;96:887-92 pubmed

product information

Product Type :

Antibody

Product Name :

6x-His Tag (C-term) Monoclonal Antibody (3D5), FITC

Catalog # :

R933-25

Quantity :

50 uL

Price :

US 425.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Tag

Applications :

Immunocytochemistry: 1:100-1:500, Western Blot: 1:1,000

Species :

Tag

Clone :

3D5

Isotype :

IgG2b

Storage :

4 C, store in dark

Description :

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Immunogen :

6x His synthetic peptide: C-term -His-His-His-His-His-His-COOH

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:500, Western Blot: 1:1,000

Aliases :

6His tag; 6X His; His Tag; Histidine Tag