6x-His Tag (C-term) Monoclonal Antibody (3D5), AP | Invitrogen R932-25 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

6x-His Tag (C-term) Monoclonal Antibody (3D5), AP

catalog :

R932-25

quantity :

125 uL

price :

US 413.00

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

3D5

application :

western blot

citations: 7

Reference
Jaron M, Lehky M, Zar xe0 M, Zaydowicz C, Lak A, Ballmann R, et al. Baculovirus-Free SARS-CoV-2 Virus-like Particle Production in Insect Cells for Rapid Neutralization Assessment. Viruses. 2022;14: pubmed publisher
Sun P, Li X, Dilokpimol A, Henrissat B, de Vries R, Kabel M, et al. Fungal glycoside hydrolase family 44 xyloglucanases are restricted to the phylum Basidiomycota and show a distinct xyloglucan cleavage pattern. iScience. 2022;25:103666 pubmed publisher
Chen G, Cleary J, Asenjo A, Chen Y, Mascaro J, Arginteanu D, et al. Kinesin-5 Promotes Microtubule Nucleation and Assembly by Stabilizing a Lattice-Competent Conformation of Tubulin. Curr Biol. 2019;29:2259-2269.e4 pubmed publisher
Janek T, Dobrowolski A, Biegalska A, Mironczuk A. Characterization of erythrose reductase from Yarrowia lipolytica and its influence on erythritol synthesis. Microb Cell Fact. 2017;16:118 pubmed publisher
Vidhyasagar V, He Y, Guo M, Ding H, Talwar T, Nguyen V, et al. C-termini are essential and distinct for nucleic acid binding of human NABP1 and NABP2. Biochim Biophys Acta. 2016;1860:371-83 pubmed publisher
Ambavaram M, Basu S, Krishnan A, Ramegowda V, Batlang U, Rahman L, et al. Coordinated regulation of photosynthesis in rice increases yield and tolerance to environmental stress. Nat Commun. 2014;5:5302 pubmed publisher
Chen Z, Jing C, Gallagher S, Sheetz M, Cornish V. Second-generation covalent TMP-tag for live cell imaging. J Am Chem Soc. 2012;134:13692-9 pubmed publisher

product information

Product Type :

Antibody

Product Name :

6x-His Tag (C-term) Monoclonal Antibody (3D5), AP

Catalog # :

R932-25

Quantity :

125 uL

Price :

US 413.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Tag

Applications :

Western Blot: 1:1,000-1:5,000

Species :

Tag

Clone :

3D5

Isotype :

IgG2b

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Immunogen :

6x His synthetic peptide: C-term -His-His-His-His-His-His-COOH

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1:1,000-1:5,000

Aliases :

6His tag; 6X His; His Tag; Histidine Tag