6x-His Tag (C-term) Monoclonal Antibody (3D5), HRP | Invitrogen R931-25 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

6x-His Tag (C-term) Monoclonal Antibody (3D5), HRP

catalog :

R931-25

quantity :

50 uL

price :

US 404.00

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

3D5

application :

western blot, immunoprecipitation

citations: 33

Reference
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Zhao H, To K, Lam H, Zhang C, Peng Z, Meng X, et al. A trifunctional peptide broadly inhibits SARS-CoV-2 Delta and Omicron variants in hamsters. Cell Discov. 2022;8:62 pubmed publisher
Qin X, Duan M, Pei D, Lin J, Wang L, Zhou P, et al. Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon α2b. J Pharm Anal. 2022;12:308-316 pubmed publisher
Zhao H, Meng X, Peng Z, Lam H, Zhang C, Zhou X, et al. Fusion-inhibition peptide broadly inhibits influenza virus and SARS-CoV-2, including Delta and Omicron variants. Emerg Microbes Infect. 2022;11:926-937 pubmed publisher
Soo M, Saltzman A. Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays. Bio Protoc. 2021;11:e4168 pubmed publisher
Zhao H, To K, Sze K, Yung T, Bian M, Lam H, et al. A broad-spectrum virus- and host-targeting peptide against respiratory viruses including influenza virus and SARS-CoV-2. Nat Commun. 2020;11:4252 pubmed publisher
Haeger W, Henning J, Heckel D, Pauchet Y, Kirsch R. Direct evidence for a new mode of plant defense against insects via a novel polygalacturonase-inhibiting protein expression strategy. J Biol Chem. 2020;295:11833-11844 pubmed publisher
Ki xdf ling L, Schneider C, Seibel K, Dorjjugder N, Busche T, Kalinowski J, et al. The roseoflavin producer Streptomyces davaonensis has a high catalytic capacity and specific genetic adaptations with regard to the biosynthesis of riboflavin. Environ Microbiol. 2020;22:3248-3265 pubmed publisher
Yuh D, Maekawa T, Li X, Kajikawa T, Bdeir K, Chavakis T, et al. The secreted protein DEL-1 activates a β3 integrin-FAK-ERK1/2-RUNX2 pathway and promotes osteogenic differentiation and bone regeneration. J Biol Chem. 2020;295:7261-7273 pubmed publisher
Elmer B, Swanson K, Bangari D, Piepenhagen P, Roberts E, Taksir T, et al. Gene delivery of a modified antibody to Aβ reduces progression of murine Alzheimer's disease. PLoS ONE. 2019;14:e0226245 pubmed publisher
Conter C, Oppici E, Dindo M, Rossi L, Magnani M, Cellini B. Biochemical properties and oxalate-degrading activity of oxalate decarboxylase from bacillus subtilis at neutral pH. IUBMB Life. 2019;71:917-927 pubmed publisher
Cartwright T, Worrell J, Marchetti L, Dowling C, Knox A, Kiely P, et al. HDAC1 interacts with the p50 NF-?B subunit via its nuclear localization sequence to constrain inflammatory gene expression. Biochim Biophys Acta Gene Regul Mech. 2018;1861:962-970 pubmed publisher
Deng L, Chang T, Wang Y, Li S, Wang S, Matsuyama S, et al. Heterosubtypic influenza protection elicited by double-layered polypeptide nanoparticles in mice. Proc Natl Acad Sci U S A. 2018;115:E7758-E7767 pubmed publisher
Zhao H, To K, Chu H, Ding Q, Zhao X, Li C, et al. Dual-functional peptide with defective interfering genes effectively protects mice against avian and seasonal influenza. Nat Commun. 2018;9:2358 pubmed publisher
Deng L, Mohan T, Chang T, Gonzalez G, Wang Y, Kwon Y, et al. Double-layered protein nanoparticles induce broad protection against divergent influenza A viruses. Nat Commun. 2018;9:359 pubmed publisher
Chen Y, Wu K, Wu K, Wu K, Tsai H, Chen M, et al. Recombinant Adeno-Associated Virus-Mediated Expression of Methamphetamine Antibody Attenuates Methamphetamine-Induced Hyperactivity in Mice. Sci Rep. 2017;7:46301 pubmed publisher
Verstraete K, Peelman F, Braun H, Lopez J, Van Rompaey D, Dansercoer A, et al. Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma. Nat Commun. 2017;8:14937 pubmed publisher
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Horvath M, Mihajlovic Z, Slaninova V, Perez Gomez R, Moshkin Y, Krejci A. The silent information regulator 1 (Sirt1) is a positive regulator of the Notch pathway in Drosophila. Biochem J. 2016;473:4129-4143 pubmed
Hafver T, Hodne K, Wanichawan P, Aronsen J, Dalhus B, Lunde P, et al. Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman. J Biol Chem. 2016;291:4561-79 pubmed publisher
Wilson K, Mole D, Binnie M, Homer N, Zheng X, Yard B, et al. Bacterial expression of human kynurenine 3-monooxygenase: solubility, activity, purification. Protein Expr Purif. 2014;95:96-103 pubmed publisher
Domenighetti A, Chu P, Wu T, Sheikh F, Gokhin D, Guo L, et al. Loss of FHL1 induces an age-dependent skeletal muscle myopathy associated with myofibrillar and intermyofibrillar disorganization in mice. Hum Mol Genet. 2014;23:209-25 pubmed publisher
Perez Leal O, Barrero C, Merali S. Translational control of Nrf2 within the open reading frame. Biochem Biophys Res Commun. 2013;437:134-9 pubmed publisher
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product information

Product Type :

Antibody

Product Name :

6x-His Tag (C-term) Monoclonal Antibody (3D5), HRP

Catalog # :

R931-25

Quantity :

50 uL

Price :

US 404.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Tag

Applications :

Western Blot: 1:1,000-1:5,000

Species :

Tag

Clone :

3D5

Isotype :

IgG2b

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Immunogen :

C-term 6x His synthetic peptide: -His-His-His-His-His-His-COOH

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1:1,000-1:5,000

Aliases :

6His tag; 6X His; His Tag; Histidine Tag