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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
6x-His Tag Monoclonal Antibody (3D5)
catalog :
R930-25
quantity :
50 µL
price :
US 329.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3D5
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, other
more info or order :
Invitrogen product webpage
citations: 38
Reference
Wu Y, Mahtal N, Paillares E, Swistak L, Sagadiev S, Acharya M, et al. C910 chemical compound inhibits the traffiking of several bacterial AB toxins with cross-protection against influenza virus. iScience. 2022;25:104537 pubmed publisher
Sun X, Liu Y, Huang Z, Xu W, Hu W, Yi L, et al. SARS-CoV-2 non-structural protein 6 triggers NLRP3-dependent pyroptosis by targeting ATP6AP1. Cell Death Differ. 2022;29:1240-1254 pubmed publisher
Phillips A, Lawrence K, Moulana A, Dupic T, Chang J, Johnson M, et al. Binding affinity landscapes constrain the evolution of broadly neutralizing anti-influenza antibodies. elife. 2021;10: pubmed publisher
Tapia D, Sanchez Villamil J, Torres A. Multicomponent gold nano-glycoconjugate as a highly immunogenic and protective platform against Burkholderia mallei. NPJ Vaccines. 2020;5:82 pubmed publisher
Pemmari T, Ivanova L, May U, Lingasamy P, Tobi A, Pasternack A, et al. Exposed CendR Domain in Homing Peptide Yields Skin-Targeted Therapeutic in Epidermolysis Bullosa. Mol Ther. 2020;28:1833-1845 pubmed publisher
Scott E, Jacobus E, Lyons B, FROST S, Freedman J, Dyer A, et al. Bi- and tri-valent T cell engagers deplete tumour-associated macrophages in cancer patient samples. J Immunother Cancer. 2019;7:320 pubmed publisher
Mravic M, Hu H, Lu Z, Bennett J, Sanders C, Orr A, et al. De novo designed transmembrane peptides activating the α5β1 integrin. Protein Eng Des Sel. 2018;31:181-190 pubmed publisher
Wang J, Hilchey S, DeDiego M, Perry S, Hyrien O, Nogales A, et al. Broad cross-reactive IgG responses elicited by adjuvanted vaccination with recombinant influenza hemagglutinin (rHA) in ferrets and mice. PLoS ONE. 2018;13:e0193680 pubmed publisher
Virant D, Traenkle B, Maier J, Kaiser P, Bodenhöfer M, Schmees C, et al. A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging. Nat Commun. 2018;9:930 pubmed publisher
Bednenko J, Harriman R, Mariën L, Nguyen H, Agrawal A, Papoyan A, et al. A multiplatform strategy for the discovery of conventional monoclonal antibodies that inhibit the voltage-gated potassium channel Kv1.3. MAbs. 2018;10:636-650 pubmed publisher
Chen Y, Farzadfard F, Gharaei N, Chen W, Cao J, Lu T. Randomized CRISPR-Cas Transcriptional Perturbation Screening Reveals Protective Genes against Alpha-Synuclein Toxicity. Mol Cell. 2017;68:247-257.e5 pubmed publisher
Lucena Araujo A, Coelho Silva J, Pereira Martins D, Thomé C, Scheucher P, Lange A, et al. ΔNp73 overexpression promotes resistance to apoptosis but does not cooperate with PML/RARA in the induction of an APL-leukemic phenotype. Oncotarget. 2017;8:8475-8483 pubmed publisher
Lord M, Cheng B, Tang F, Lyons J, Rnjak Kovacina J, Whitelock J. Bioengineered human heparin with anticoagulant activity. Metab Eng. 2016;38:105-114 pubmed publisher
Clow K, Short C, Hall J, Gendron R, Paradis H, Ralhan A, et al. High rates of glucose utilization in the gas gland of Atlantic cod (Gadus morhua) are supported by GLUT1 and HK1b. J Exp Biol. 2016;219:2763-73 pubmed publisher
Cho S, Contreras L, Ju S. Synthetic chimeras with orthogonal ribosomal proteins increase translation yields by recruiting mRNA for translation as measured by profiling active ribosomes. Biotechnol Prog. 2016;32:285-93 pubmed publisher
Fonteneau J, Brilot F, Münz C, Gannagé M. The Tumor Antigen NY-ESO-1 Mediates Direct Recognition of Melanoma Cells by CD4+ T Cells after Intercellular Antigen Transfer. J Immunol. 2016;196:64-71 pubmed publisher
Arpağ G, Shastry S, Hancock W, Tüzel E. Transport by populations of fast and slow kinesins uncovers novel family-dependent motor characteristics important for in vivo function. Biophys J. 2014;107:1896-1904 pubmed publisher
Pante N, Fahrenkrog B. Exploring nuclear pore complex molecular architecture by immuno-electron microscopy using Xenopus oocytes. Methods Cell Biol. 2014;122:81-98 pubmed publisher
Li J, Arévalo M, Chen Y, Posadas O, Smith J, Zeng M. Intranasal immunization with influenza antigens conjugated with cholera toxin subunit B stimulates broad spectrum immunity against influenza viruses. Hum Vaccin Immunother. 2014;10:1211-20 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
6x-His Tag Monoclonal Antibody (3D5)
Catalog # :
R930-25
Quantity :
50 µL
Price :
US 329.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Tag
Applications :
ELISA: Assay-dependent, Immunocytochemistry: 1:100-1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:5,000
Species :
Tag
Clone :
3D5
Isotype :
IgG2b
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Immunogen :
6x His synthetic peptide
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunocytochemistry: 1:100-1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:5,000
Aliases :
6His tag; 6X His; His Tag; Histidine Tag
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA