POLQ Polyclonal Antibody | Invitrogen PA5-69577 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

POLQ Polyclonal Antibody

catalog :

PA5-69577

quantity :

100 uL

price :

US 454.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

citations: 3

Reference
Hu C, Bugbee T, Palinski R, Akinyemi I, McIntosh M, MacCarthy T, et al. Beta human papillomavirus 8E6 promotes alternative end joining. elife. 2023;12: pubmed publisher
Bai H, Xia S, Zhu L, Dong Y, Liu C, Li N, et al. Altered polymerase theta expression promotes chromosomal instability in salivary adenoid cystic carcinoma. J Cell Mol Med. 2022;: pubmed publisher
Black S, Ozdemir A, Kashkina E, Kent T, Rusanov T, Ristic D, et al. Molecular basis of microhomology-mediated end-joining by purified full-length Polθ. Nat Commun. 2019;10:4423 pubmed publisher

product information

Product Type :

Antibody

Product Name :

POLQ Polyclonal Antibody

Catalog # :

PA5-69577

Quantity :

100 uL

Price :

US 454.00

Clonality :

Polyclonal

Purity :

Affinity chromatography

Host :

Rabbit

Reactivity :

Human

Applications :

Western Blot: 1.0 ug/mL

Species :

Human

Isotype :

IgG

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs. [UniProt]

Immunogen :

synthetic peptide directed towards the C terminal region of human POLQ

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1.0 ug/mL

Aliases :

A430110D14Rik; Chaos1; Chromosome aberrations occurring spontaneously protein 1; DNA polymerase eta; DNA polymerase theta; DNA polymerase theta; LOW QUALITY PROTEIN: DNA polymerase theta; EC 2.7.7.7; POLH; Polq; polymerase (DNA directed), theta; polymerase (DNA) theta; PRO0327