Nanog Polyclonal Antibody | Invitrogen PA5-47376 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Nanog Polyclonal Antibody

catalog :

PA5-47376

quantity :

100 ug

price :

US 454.00

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, immunocytochemistry, flow cytometry

product information

Product Type :

Antibody

Product Name :

Nanog Polyclonal Antibody

Catalog # :

PA5-47376

Quantity :

100 ug

Price :

US 454.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Goat

Reactivity :

Mouse

Applications :

Flow Cytometry: 0.25 ug per million cells, Immunocytochemistry: 5-15 ug/mL, Western Blot: Assay-Dependent

Species :

Mouse

Isotype :

IgG

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

Immunogen :

E. coli-derived recombinant mouse Nanog Trp154-Leu262

Format :

Lyophilized

Applications w/Dilutions :

Flow Cytometry: 0.25 ug per million cells, Immunocytochemistry: 5-15 ug/mL, Western Blot: Assay-Dependent

Aliases :

2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox