TNF alpha Polyclonal Antibody | Invitrogen PA5-46945 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TNF alpha Polyclonal Antibody

catalog :

PA5-46945

quantity :

100 ug

price :

US 514.00

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200; loading ...; fig 7	Drake L, Brooks A, Stauff J, Sherman P, Arteaga J, Koeppe R, et al. Strategies for PET imaging of the receptor for advanced glycation endproducts (RAGE). J Pharm Anal. 2020;10:452-465 pubmed publisher
immunohistochemistry; mouse; 1:40; loading ...; fig 5c	LeBlang C, Medalla M, Nicoletti N, Hays E, Zhao J, Shattuck J, et al. Reduction of the RNA Binding Protein TIA1 Exacerbates Neuroinflammation in Tauopathy. Front Neurosci. 2020;14:285 pubmed publisher
	Ziegler Waldkirch S, Friesen M, Loreth D, Sauer J, Kemna S, Hilse A, et al. Seed-induced Aβ deposition alters neuronal function and impairs olfaction in a mouse model of Alzheimer's disease. Mol Psychiatry. 2022;27:4274-4284 pubmed publisher
	Bruno M, Mukherjee S, Stromberg A, Saito H, Starr M. Visceral fat-specific regulation of plasminogen activator inhibitor-1 in aged septic mice. J Cell Physiol. 2022;237:706-719 pubmed publisher

product information

Product Type :

Antibody

Product Name :

TNF alpha Polyclonal Antibody

Catalog # :

PA5-46945

Quantity :

100 ug

Price :

US 514.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Goat

Reactivity :

Human, Mouse

Applications :

ELISA: 0.1-0.4 ug/mL (Detection), 0.2-0.8 ug/mL (Capture), Flow Cytometry: 2.5 ug per million cells, Immunocytochemistry: 5-15 ug/mL, Immunohistochemistry (Paraffin): 3-15 ug/mL, Neutralization: 0.1-0.4 ug/mL, Western Blot: 2 ug/mL

Species :

Human, Mouse

Isotype :

IgG

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Immunogen :

E. coli-derived recombinant mouse TNF-alpha Leu80-Leu235

Format :

Lyophilized

Applications w/Dilutions :

Aliases :

APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor

more info or order :

Invitrogen product webpage