PST1 Polyclonal Antibody | Invitrogen PA5-34414 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PST1 Polyclonal Antibody

catalog :

PA5-34414

quantity :

100 ug

price :

US 464.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

Saccharomyces cerevisiae S288C

application :

western blot, ELISA

product information

Product Type :

Antibody

Product Name :

PST1 Polyclonal Antibody

Catalog # :

PA5-34414

Quantity :

100 ug

Price :

US 464.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Yeast

Applications :

ELISA: 1 ug/mL, Western Blot: 1 ug/mL

Species :

Yeast

Isotype :

IgG

Storage :

Maintain refrigerated at 2-8 C for up to 3 months. For long term storage store at -20 C

Description :

Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain, which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.

Immunogen :

A synthetic peptide from the amino terminus of yeast Pst1

Format :

Liquid

Applications w/Dilutions :

ELISA: 1 ug/mL, Western Blot: 1 ug/mL

Aliases :

Cell wall mannoprotein PST1; D4214; Haze protective factor 2; HPF2; Protoplast secreted protein 1; PST1; Pst1p; YD9609.09; YDR055W