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antibody

UFD1L Polyclonal Antibody

PA5-31114

100 uL

US 464.00

polyclonal

domestic rabbit

nonconjugated

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

UFD1L Polyclonal Antibody

PA5-31114

100 uL

US 464.00

Polyclonal

Antigen affinity chromatography

Rabbit

Human

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Human

IgG

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Ubiquitin-mediated proteolysis requires the transfer of ubiquitin (Ub) to lysine groups on selected cellular proteins, which then potentiates the proteolytic degradation of these protein conjugates by the 26S proteasome. Ub-fusions are cleaved by Ub-specific processing proteases (UBps) or alternatively by the Ub-fusion degradation (UFD) pathway. The UBP pathway targets the C-terminal glycine residue on Ub that is involved in the formation of Ub-conjugates, while UFD proteins preferentially cleave Ub-conjugated proteins that contain an amino acid substitution at this glycine residue. The UFD1 protein was originally characterized in the yeast S. cerevisiae and subsequently, the human homolog UFD1 or UFD1L was identified. In vitro, UFD1 attenuates the degradation of Ub-fusions, which have a proline or valine residue substituted at the Gly76 moiety, by the selective multiubiquitination of the Ub chain of the Ub-conjugate. Mutations within the UFD1 gene are implicated in the development of CATCH22 syndrome, which is characterized by cardiac defects, cleft palate and hypocalcemia, suggesting that this proteolytic pathway may be involved in the progression of these developmental defects.

Recombinant fragment corresponding to a region within amino acids 45 and 307 of Human UFD1L

Liquid

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

RGD:619822}; UB fusion protein 1; UB fusion protein 1 {ECO:0000250; ubiquitin fusion degradation 1 like; ubiquitin fusion degradation 1 like (yeast); Ubiquitin fusion degradation protein 1; Ubiquitin fusion degradation protein 1 homolog; ubiquitin fusion degradation protein 1 homolog {ECO:0000250; Ubiquitin recognition factor in ER-associated degradation protein 1; UFD1; UFD1L; ufd1l {ECO:0000312; UniProtKB:Q92890}