NSE Polyclonal Antibody | Invitrogen PA5-27452 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NSE Polyclonal Antibody

catalog :

PA5-27452

quantity :

100 µL

price :

US 514

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:500; fig 4	Waldeck K, Cullinane C, Ardley K, Shortt J, Martin B, Tothill R, et al. Long term, continuous exposure to panobinostat induces terminal differentiation and long term survival in the TH-MYCN neuroblastoma mouse model. Int J Cancer. 2016;139:194-204 pubmed publisher
	Mustafa F. Characterization of giant neuroendocrine cells in the pregnant New Zealand white rabbit vagina demonstrated by histological, histochemical, and immunohistochemical methods. Microsc Res Tech. 2022;85:3309-3315 pubmed publisher
	Mustafa F, Abdelhafez E, Abd Elhafeez H. Characterization of the primo vascular system in rabbit vagina. Microsc Res Tech. 2022;85:799-806 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded PC14 xenograft using ENO2 (PA5-27452) antibody at 1:100 dilution.

image 2 :

Western Blot analysis of ENO2 using anti-ENO2 Polyclonal Antibody (PA5-27452), in Mouse Brain (50 ug of cell lysate) and at a dilution of 1:10000.

image 3 :

Western Blot analysis using anti-ENO2 Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A:A431 cell lysate B:H1299. Primary antibody (PA5-27452) diluted at 1:2000

product information

Product Type :

Antibody

Product Name :

NSE Polyclonal Antibody

Catalog # :

PA5-27452

Quantity :

100 µL

Price :

US 514

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunohistochemistry (Frozen): 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:1,000-1:10,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.NSE is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The alpha-subunit is expressed in most tissues, the beta-subunit only in muscle, and the gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to NSE protein are useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Further, NSE is found in elevated concentrations in plasma and certain neoplasias that include pediatric neuroblastoma and small cell lung cancer.

Immunogen :

Recombinant fragment corresponding to a region within amino acids 1 and 268 of Human ENO2

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Frozen): 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:1,000-1:10,000

Aliases :

2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; AI837106; D6Ertd375e; Eno; ENO2; Eno-2; ENOG; Enolase 2; enolase 2 (gamma, neuronal); enolase 2, gamma neuronal; enolase 2, gamma, neuronal; epididymis secretory protein Li 279; gamma enolase; gamma-enolase; gamma-enolase-like; gamma-subunit of enolase; HEL-S-279; LOC100911625; Neural enolase; neuron specific enolase; neuron specific gamma enolase; Neuronal Enolase; neuronal enriched enolase; neurone-specific enolase; neuron-specific enolase; NSE; RNEN3

more info or order :

Invitrogen product webpage