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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
IBA1 Polyclonal Antibody
catalog :
PA5-27436
quantity :
100 µL
price :
US 514
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 55
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:2000; loading ...; fig 2a
Hülskötter K, Luhder F, Flügel A, Herder V, Baumgartner W. Tamoxifen Application Is Associated with Transiently Increased Loss of Hippocampal Neurons following Virus Infection. Int J Mol Sci. 2021;22: pubmed publisher
  • immunohistochemistry; mouse; 1:75; loading ...; fig 7c
  • western blot; mouse; 1:1000; loading ...; fig 7c
Zahedi K, Brooks M, Barone S, Rahmati N, Murray Stewart T, Dunworth M, et al. Ablation of polyamine catabolic enzymes provokes Purkinje cell damage, neuroinflammation, and severe ataxia. J Neuroinflammation. 2020;17:301 pubmed publisher
  • immunohistochemistry; rat; 1:200; loading ...; fig 4d
Tomassoni D, Martinelli I, Moruzzi M, Micioni Di Bonaventura M, Cifani C, Amenta F, et al. Obesity and Age-Related Changes in the Brain of the Zucker Lepr fa/fa Rats. Nutrients. 2020;12: pubmed publisher
  • western blot; rat; 1:2400; fig 6
Lee Kubli C, Ingves M, Henry K, Shiao R, Collyer E, Tuszynski M, et al. Analysis of the behavioral, cellular and molecular characteristics of pain in severe rodent spinal cord injury. Exp Neurol. 2016;278:91-104 pubmed publisher
Novakovic M, Korshunov K, Grant R, Martin M, Valencia H, Budinger G, et al. Astrocyte reactivity and inflammation-induced depression-like behaviors are regulated by Orai1 calcium channels. Nat Commun. 2023;14:5500 pubmed publisher
Grahl M, Andrade B, Perin A, Neves G, Duarte L, Uberti A, et al. Could the Urease of the Gut Bacterium Proteus mirabilis Play a Role in the Altered Gut-Brain Talk Associated with Parkinson's Disease?. Microorganisms. 2023;11: pubmed publisher
Chludzinski E, Ciurkiewicz M, Stoff M, Klemens J, Kr xfc ger J, Shin D, et al. Canine Distemper Virus Alters Defense Responses in an Ex Vivo Model of Pulmonary Infection. Viruses. 2023;15: pubmed publisher
Shi Y, Fang Y, Shen P, Liu H, Xu L, Wang L, et al. JNK-IN-8 treatment improves ARDS-induced cognitive impairment by inhibiting JNK/NF-κB-mediated NLRP3 inflammasome. Brain Behav. 2023;13:e2980 pubmed publisher
Liang J, Han S, Ye C, Zhu H, Wu J, Nie Y, et al. Minocycline Attenuates Sevoflurane-Induced Postoperative Cognitive Dysfunction in Aged Mice by Suppressing Hippocampal Apoptosis and the Notch Signaling Pathway-Mediated Neuroinflammation. Brain Sci. 2023;13: pubmed publisher
Hong N, Kim H, Kang K, Park J, Mun S, Kim H, et al. Photobiomodulation improves the synapses and cognitive function and ameliorates epileptic seizure by inhibiting downregulation of Nlgn3. Cell Biosci. 2023;13:8 pubmed publisher
Kim U, Hong N, Ahn J. Photobiomodulation Attenuated Cognitive Dysfunction and Neuroinflammation in a Prenatal Valproic Acid-Induced Autism Spectrum Disorder Mouse Model. Int J Mol Sci. 2022;23: pubmed publisher
Lee H, Choe K, Park J, Khan A, Kim M, Park T, et al. O-Cyclic Phytosphingosine-1-Phosphate Protects against Motor Dysfunctions and Glial Cell Mediated Neuroinflammation in the Parkinson's Disease Mouse Models. Antioxidants (Basel). 2022;11: pubmed publisher
Chludzinski E, Klemens J, Ciurkiewicz M, Geffers R, P xf6 pperl P, Stoff M, et al. Phenotypic and Transcriptional Changes of Pulmonary Immune Responses in Dogs Following Canine Distemper Virus Infection. Int J Mol Sci. 2022;23: pubmed publisher
Huwart S, de Wouters d Oplinter A, Rastelli M, Van Hul M, de Vos W, Luquet S, et al. Food Reward Alterations during Obesity Are Associated with Inflammation in the Striatum in Mice: Beneficial Effects of Akkermansia muciniphila. Cells. 2022;11: pubmed publisher
Chambers L, Diaz Otero J, Fisher C, Jackson W, Dorrance A. Mineralocorticoid receptor antagonism improves transient receptor potential vanilloid 4-dependent dilation of cerebral parenchymal arterioles and cognition in a genetic model of hypertension. J Hypertens. 2022;40:1722-1734 pubmed publisher
Jeon J, Kim S, Kong E, Kim S, Yang J, Lee J, et al. Establishment of the reproducible branch retinal artery occlusion mouse model and intravital longitudinal imaging of the retinal CX3CR1-GFP+ cells after spontaneous arterial recanalization. Front Med (Lausanne). 2022;9:897800 pubmed publisher
Uberti A, Callai Silva N, Grahl M, Piovesan A, Nachtigall E, Furini C, et al. Helicobacter pylori Urease: Potential Contributions to Alzheimer's Disease. Int J Mol Sci. 2022;23: pubmed publisher
Wlodarek L, Alibhai F, Wu J, Li S, Li R. Stroke-Induced Neurological Dysfunction in Aged Mice Is Attenuated by Preconditioning with Young Sca-1+ Stem Cells. Stem Cells. 2022;40:564-576 pubmed publisher
Wang S, Zaitoun I, Darjatmoko S, Sheibani N, Sorenson C. Bim Expression Promotes the Clearance of Mononuclear Phagocytes during Choroidal Neovascularization, Mitigating Scar Formation in Mice. Life (Basel). 2022;12: pubmed publisher
Mahendrasingam S, Osborn A, Israr M, Riasat K, Gater R, Spano A, et al. The effects of substrate composition and topography on the characteristics and growth of cell cultures of cochlear fibrocytes. Hear Res. 2022;415:108427 pubmed publisher
Hoshino Y, Sonoda H, Mikoda N, Ikeda M. Upregulation of NADPH Oxidase 2 Contributes to Renal Fibrosis in pcy Mice: An Experimental Model of Nephronophthisis. Nephron. 2022;146:393-403 pubmed publisher
Desale S, Chinnathambi S. α-Linolenic acid induces clearance of Tau seeds via Actin-remodeling in Microglia. Mol Biomed. 2021;2:4 pubmed publisher
Chludzinski E, Puff C, Weber J, Hewicker Trautwein M. Case Report: Primary Diffuse Leptomeningeal Oligodendrogliomatosis in a Young Adult Cat. Front Vet Sci. 2021;8:795126 pubmed publisher
Liu W, Danckwardt Lilliestr xf6 m N, Schrott Fischer A, Glueckert R, Rask Andersen H. Distribution of Immune Cells Including Macrophages in the Human Cochlea. Front Neurol. 2021;12:781702 pubmed publisher
Park M, Kim H, Shin H, Lee S, Hwang D, Lew H. Human Pluripotent Stem Cell-Derived Neural Progenitor Cells Promote Retinal Ganglion Cell Survival and Axon Recovery in an Optic Nerve Compression Animal Model. Int J Mol Sci. 2021;22: pubmed publisher
Saeed K, Jo M, Park J, Alam S, Khan I, Ahmad R, et al. 17β-Estradiol Abrogates Oxidative Stress and Neuroinflammation after Cortical Stab Wound Injury. Antioxidants (Basel). 2021;10: pubmed publisher
Xu Q, Ford N, He S, Huang Q, Anderson M, Chen Z, et al. Astrocytes contribute to pain gating in the spinal cord. Sci Adv. 2021;7:eabi6287 pubmed publisher
Conti F, Romano G, Eandi C, Toro M, Rejdak R, Di Benedetto G, et al. Brimonidine is Neuroprotective in Animal Paradigm of Retinal Ganglion Cell Damage. Front Pharmacol. 2021;12:705405 pubmed publisher
Verma D, Seo B, Ghosh A, Ma S, Hernandez Quijada K, Andersen J, et al. Alpha-Synuclein Preformed Fibrils Induce Cellular Senescence in Parkinson's Disease Models. Cells. 2021;10: pubmed publisher
Yoon S, Hong N, Lee M, Ahn J. Photobiomodulation with a 660-Nanometer Light-Emitting Diode Promotes Cell Proliferation in Astrocyte Culture. Cells. 2021;10: pubmed publisher
DORA D, Ferenczi S, Stavely R, Tóth V, Varga Z, Kovacs T, et al. Evidence of a Myenteric Plexus Barrier and Its Macrophage-Dependent Degradation During Murine Colitis: Implications in Enteric Neuroinflammation. Cell Mol Gastroenterol Hepatol. 2021;12:1617-1641 pubmed publisher
M xe4 kinen E, Lensu S, Honkanen M, Laitinen P, Wikgren J, Koch L, et al. Rats bred for low intrinsic aerobic exercise capacity link obesity with brain inflammation and reduced structural plasticity of the hippocampus. Brain Behav Immun. 2021;97:250-259 pubmed publisher
Khan I, Saeed K, Jo M, Kim M. 17-β Estradiol Rescued Immature Rat Brain against Glutamate-Induced Oxidative Stress and Neurodegeneration via Regulating Nrf2/HO-1 and MAP-Kinase Signaling Pathway. Antioxidants (Basel). 2021;10: pubmed publisher
Han Y, Xia G, Srisai D, Meng F, He Y, Ran Y, et al. Deciphering an AgRP-serotoninergic neural circuit in distinct control of energy metabolism from feeding. Nat Commun. 2021;12:3525 pubmed publisher
Iacobucci S, Padilla N, Gabrielli M, Navarro C, Lombardi M, Vicioso Mantis M, et al. The histone demethylase PHF8 regulates astrocyte differentiation and function. Development. 2021;148: pubmed publisher
Wang Y, Monteagudo A, Downey M, Ashton Rickardt P, Elmaleh D. Cromolyn inhibits the secretion of inflammatory cytokines by human microglia (HMC3). Sci Rep. 2021;11:8054 pubmed publisher
Desale S, Chinnathambi S. α- Linolenic acid modulates phagocytosis and endosomal pathways of extracellular Tau in microglia. Cell Adh Migr. 2021;15:84-100 pubmed publisher
Perez Pardo P, Grobben Y, Willemsen Seegers N, Hartog M, Tutone M, Muller M, et al. Pharmacological validation of TDO as a target for Parkinson's disease. FEBS J. 2021;288:4311-4331 pubmed publisher
Mei B, Li J, Zuo Z. Dexmedetomidine attenuates sepsis-associated inflammation and encephalopathy via central α2A adrenoceptor. Brain Behav Immun. 2021;91:296-314 pubmed publisher
Borghi S, Fattori V, Carvalho T, Tatakihara V, Zaninelli T, Pinho Ribeiro F, et al. Experimental Trypanosoma cruzi Infection Induces Pain in Mice Dependent on Early Spinal Cord Glial Cells and NFκB Activation and Cytokine Production. Front Immunol. 2020;11:539086 pubmed publisher
Saeed K, Shah S, Ullah R, Alam S, Park J, Saleem S, et al. Quinovic Acid Impedes Cholesterol Dyshomeostasis, Oxidative Stress, and Neurodegeneration in an Amyloid-β-Induced Mouse Model. Oxid Med Cell Longev. 2020;2020:9523758 pubmed publisher
Lee K, Kim J, Han S, Lee D, Lee H, Yim S, et al. The extracellular vesicle of gut microbial Paenalcaligenes hominis is a risk factor for vagus nerve-mediated cognitive impairment. Microbiome. 2020;8:107 pubmed publisher
Eikelberg D, Lehmbecker A, Brogden G, Tongtako W, Hahn K, Habierski A, et al. Axonopathy and Reduction of Membrane Resistance: Key Features in a New Murine Model of Human GM1-Gangliosidosis. J Clin Med. 2020;9: pubmed publisher
Schlepckow K, Monroe K, Kleinberger G, Cantuti Castelvetri L, Parhizkar S, Xia D, et al. Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region. EMBO Mol Med. 2020;12:e11227 pubmed publisher
Das R, Balmik A, Chinnathambi S. Phagocytosis of full-length Tau oligomers by Actin-remodeling of activated microglia. J Neuroinflammation. 2020;17:10 pubmed publisher
Stork T, Nessler J, Anderegg L, Hünerfauth E, Schmutz I, Jagannathan V, et al. TSEN54 missense variant in Standard Schnauzers with leukodystrophy. PLoS Genet. 2019;15:e1008411 pubmed publisher
Liu W, Kämpfe Nordström C, Danckwardt Lillieström N, Rask Andersen H. Human Inner Ear Immune Activity: A Super-Resolution Immunohistochemistry Study. Front Neurol. 2019;10:728 pubmed publisher
Borghi S, Fattori V, Pinho Ribeiro F, Domiciano T, Miranda Sapla M, Zaninelli T, et al. Contribution of spinal cord glial cells to L. amazonensis experimental infection-induced pain in BALB/c mice. J Neuroinflammation. 2019;16:113 pubmed publisher
Klotz D, Gerhauser I. Interferon-Stimulated Genes-Mediators of the Innate Immune Response during Canine Distemper Virus Infection. Int J Mol Sci. 2019;20: pubmed publisher
Liu W, Rask Andersen H. Super-resolution immunohistochemistry study on CD4 and CD8 cells and the relation to macrophages in human cochlea. J Otol. 2019;14:1-5 pubmed publisher
Kämpfe Nordström C, Danckwardt Lillieström N, Laurell G, Liu W, Rask Andersen H. The Human Endolymphatic Sac and Inner Ear Immunity: Macrophage Interaction and Molecular Expression. Front Immunol. 2018;9:3181 pubmed publisher
Hoshino Y, Sonoda H, Nishimura R, Mori K, Ishibashi K, Ikeda M. Involvement of the NADPH oxidase 2 pathway in renal oxidative stress in Aqp11-/- mice. Biochem Biophys Rep. 2019;17:169-176 pubmed publisher
Liu W, Molnár M, Garnham C, Benav H, Rask Andersen H. Macrophages in the Human Cochlea: Saviors or Predators-A Study Using Super-Resolution Immunohistochemistry. Front Immunol. 2018;9:223 pubmed publisher
Liu Z, Han T, Werner H, Rosen C, Schaffler M, Yakar S. Reduced Serum IGF-1 Associated With Hepatic Osteodystrophy Is a Main Determinant of Low Cortical but Not Trabecular Bone Mass. J Bone Miner Res. 2018;33:123-136 pubmed publisher
Ostapchenko V, Chen M, Guzman M, Xie Y, Lavine N, Fan J, et al. The Transient Receptor Potential Melastatin 2 (TRPM2) Channel Contributes to β-Amyloid Oligomer-Related Neurotoxicity and Memory Impairment. J Neurosci. 2015;35:15157-69 pubmed publisher
image
image 1 :
Invitrogen PA5-27436 image 1
Flow cytometry on primary murine microglia cells staining with Iba1 (PA5-27436) antibody using 1.0 µg per 4×105 cells. PA5-27436 (blue) Rabbit IgG (green) Unstained (red).
image 2 :
Invitrogen PA5-27436 image 2
Western Blot analysis of Iba1 using anti-Iba1 Polyclonal Antibody (PA5-27436), in Mouse Liver (50 ug of cell lysate) and at a dilution of 1:1000.
image 3 :
Invitrogen PA5-27436 image 3
Western Blot analysis using anti-Iba1 Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: THP-1 B: HL-60. Primary antibody (PA5-27436) diluted at 1:5000
product information
Product Type :
Antibody
Product Name :
IBA1 Polyclonal Antibody
Catalog # :
PA5-27436
Quantity :
100 µL
Price :
US 514
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Human, Mouse, Rat
Applications :
Flow Cytometry: 1:50-1:200, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:500-1:10,000
Species :
Human, Mouse, Rat
Isotype :
IgG
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
AIF1 is induced by cytokines and interferon. It is thought to be involved in the negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
Immunogen :
Recombinant protein encompassing a sequence within the center region of human Iba1
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1:50-1:200, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:500-1:10,000
Aliases :
AI607846; AIF1; AIF-1; Allograft inflammatory factor 1; balloon angioplasty responsive transcript; balloon angioplasty-responsive transcript 1; Bart1; BART-1; D17H6S50E; DADB-70P7.8; DASS-82G15.2; Em:AF129756.17; G1; Iba; Iba1; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule 1; IRT1; IRT-1; microglia response factor; Mrf1; MRF-1; protein BART-1; protein G1; testis specific
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA