LDHA Polyclonal Antibody | Invitrogen PA5-27406 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

LDHA Polyclonal Antibody

catalog :

PA5-27406

quantity :

100 µL

price :

US 514

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; fig 5a	Singh L, Devi T. Potential Combination Drug Therapy to Prevent Redox Stress and Mitophagy Dysregulation in Retinal Müller Cells under High Glucose Conditions: Implications for Diabetic Retinopathy. Diseases. 2021;9: pubmed publisher
	Abu El Maaty M, Terzic J, Keime C, Rovito D, Lutzing R, Yanushko D, et al. Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression. Sci Adv. 2022;8:eabo2295 pubmed publisher
	Xu B, Li F, Zhang W, Su Y, Tang L, Li P, et al. Identification of metabolic pathways underlying FGF1 and CHIR99021-mediated cardioprotection. iScience. 2022;25:104447 pubmed publisher
	Harrison M, Hochreiner E, Benjamin B, Lawler S, Zwezdaryk K. Metabolic Reprogramming of Glioblastoma Cells during HCMV Infection Induces Secretome-Mediated Paracrine Effects in the Microenvironment. Viruses. 2022;14: pubmed publisher
	Li P, Li F, Tang L, Zhang W, Jin Y, Gu H, et al. Metabolic Profile in Neonatal Pig Hearts. Front Cardiovasc Med. 2021;8:763984 pubmed publisher
	D Onofrio N, Martino E, Balestrieri A, Mele L, Neglia G, Balestrieri M, et al. SIRT3 and Metabolic Reprogramming Mediate the Antiproliferative Effects of Whey in Human Colon Cancer Cells. Cancers (Basel). 2021;13: pubmed publisher
	Minami N, Tanaka K, Sasayama T, Kohmura E, Saya H, Sampetrean O. Lactate Reprograms Energy and Lipid Metabolism in Glucose-Deprived Oxidative Glioma Stem Cells. Metabolites. 2021;11: pubmed publisher
	Wang D, Hartman R, Han C, Zhou C, Couch B, Malkamaki M, et al. Lactate oxidative phosphorylation by annulus fibrosus cells: evidence for lactate-dependent metabolic symbiosis in intervertebral discs. Arthritis Res Ther. 2021;23:145 pubmed publisher
	Arra M, Swarnkar G, Ke K, Otero J, Ying J, Duan X, et al. LDHA-mediated ROS generation in chondrocytes is a potential therapeutic target for osteoarthritis. Nat Commun. 2020;11:3427 pubmed publisher

image

image 1 :

Immunofluorescence analysis of methanol-fixed A431 cells using Lactate Dehydrogenase (PA5-27406) antibody at 1:500 dilution.

image 2 :

Western Blot analysis of Lactate Dehydrogenase using anti-Lactate Dehydrogenase Polyclonal Antibody (PA5-27406), in A431 Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.

image 3 :

Immunohistochemical analysis of paraffin-embedded Lung papillory AdCA using Lactate Dehydrogenase (PA5-27406) antibody at 1:500 dilution.

product information

Product Type :

Antibody

Product Name :

LDHA Polyclonal Antibody

Catalog # :

PA5-27406

Quantity :

100 µL

Price :

US 514

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

Immunogen :

Recombinant fragment corresponding to a region within amino acids 133 and 332 of Human Lactate Dehydrogenase

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Aliases :

Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; GSD11; HEL-S-133P; l7R2; Lactate Dehydrogenase; lactate dehydrogenase 1, A chain; lactate dehydrogenase 3, C chain, sperm specific; lactate dehydrogenase A; lactate dehydrogenase A4; lactate dehydrogenase A-like; lactate dehydrogenase C; lactate dehydrogenase C variant 3; lactate dehydrogenase M; lactate dehydrogenase-A; lactate dehydrogenase-M; lactate dehydrogenase-M (EC 1.1.1.27); Lactic Dehydrogenase; LDH; LDH muscle subunit; LDH testis subunit; Ldh1; Ldh-1; Ldh3; Ldha; LDH-A; ldha protein; ldha1; LDH-A4 muscle-specific isozyme; glycolytic enzyme; Ldhc; LDH-C; Ldhm; LDH-M; LDH-X; L-lactate dehydrogenase; L-lactate dehydrogenase A chain; L-lactate dehydrogenase C chain; M-LDH; OTTHUMP00000165206; PIG19; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59

more info or order :

Invitrogen product webpage