MIF Polyclonal Antibody | Invitrogen PA5-27343 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

MIF Polyclonal Antibody

catalog :

PA5-27343

quantity :

100 µL

price :

US 470

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:1200; loading ...; fig 5b western blot; mouse; 1:3000; loading ...; fig 5a	Vignjević Petrinović S, Budec M, Marković D, Gotic M, Mitrović Ajtić O, Mojsilovic S, et al. Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis. Histochem Cell Biol. 2016;146:311-24 pubmed publisher
	Guan D, Li Y, Cui Y, Zhao H, Dong N, Wang K, et al. 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction. Acta Biochim Biophys Sin (Shanghai). 2023;55:1222-1233 pubmed publisher
	Yamamoto Y, Kadoya K, Terkawi M, Endo T, Konno K, Watanabe M, et al. Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchyma. Life Sci Alliance. 2022;5: pubmed publisher
	Caltabiano R, De Pasquale R, Piombino E, Campo G, Nicoletti F, Cavalli E, et al. Macrophage Migration Inhibitory Factor (MIF) and Its Homologue d-Dopachrome Tautomerase (DDT) Inversely Correlate with Inflammation in Discoid Lupus Erythematosus. Molecules. 2021;26: pubmed publisher
	Loreto C, Caltabiano R, Graziano A, Castorina S, Lombardo C, Filetti V, et al. Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1. Eur J Histochem. 2020;64: pubmed publisher
	Farr L, Ghosh S, Jiang N, Watanabe K, Parlak M, Bucala R, et al. CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing. Cell Mol Gastroenterol Hepatol. 2020;10:101-112 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded SW480 xenograft using MIF (PA5-27343) antibody at 1:500 dilution.

image 2 :

Immunofluorescence analysis of methanol-fixed HeLa cells using MIF (PA5-27343) antibody at 1:200 dilution.

image 3 :

Western Blot analysis of MIF using anti-MIF Polyclonal Antibody (PA5-27343), in NIH-3T3 Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.

product information

Product Type :

Antibody

Product Name :

MIF Polyclonal Antibody

Catalog # :

PA5-27343

Quantity :

100 µL

Price :

US 470

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 1:1,000-1:10,000, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

Immunogen :

Recombinant fragment corresponding to a region within amino acids 1 and 115 of Human MIF

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:1,000-1:10,000, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Aliases :

cytokine; delayed early response protein 6; DER6; GIF; GLIF; glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MGC107654; Mif; MMIF; phenylpyruvate tautomerase

more info or order :

Invitrogen product webpage