Angiopoietin 2 Polyclonal Antibody | Invitrogen PA5-27297 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Angiopoietin 2 Polyclonal Antibody

catalog :

PA5-27297

quantity :

100 µL

price :

US 470

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:200; fig 6	Scholz A, Harter P, Cremer S, Yalcin B, Gurnik S, Yamaji M, et al. Endothelial cell-derived angiopoietin-2 is a therapeutic target in treatment-naive and bevacizumab-resistant glioblastoma. EMBO Mol Med. 2016;8:39-57 pubmed publisher
	Smeda M, Jasztal A, Maleki E, Bar A, Sternak M, Kwiatkowski G, et al. Endothelial-mesenchymal transition induced by metastatic 4T1 breast cancer cells in pulmonary endothelium in aged mice. Front Mol Biosci. 2022;9:1050112 pubmed publisher
	Smeda M, Stojak M, Przyborowski K, Sternak M, Suraj Prazmowska J, Kus K, et al. Direct Thrombin Inhibitor Dabigatran Compromises Pulmonary Endothelial Integrity in a Murine Model of Breast Cancer Metastasis to the Lungs; the Role of Platelets and Inflammation-Associated Haemostasis. Front Pharmacol. 2022;13:834472 pubmed publisher
	Bernatz S, Ilina E, Devraj K, Harter P, Mueller K, Kleber S, et al. Impact of Docetaxel on blood-brain barrier function and formation of breast cancer brain metastases. J Exp Clin Cancer Res. 2019;38:434 pubmed publisher
	Pedrosa A, Bodrug N, Gómez Escudero J, Carter E, Reynolds L, Georgiou P, et al. Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861. Cancer Res. 2019;: pubmed publisher
	Smeda M, Kieronska A, Proniewski B, Jasztal A, Selmi A, Wandzel K, et al. Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour. Oncotarget. 2018;9:17810-17824 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded Colon CA using Angiopoietin 2 (PA5-27297) antibody at 1:250 dilution.

image 2 :

Western Blot analysis of Angiopoietin 2 using anti-Angiopoietin 2 Polyclonal Antibody (PA5-27297), in Hep G2 Cell Lysate (30 ug of cell lysate) and at a dilution of 1:5000.

image 3 :

Western Blot analysis of Angiopoietin 2 using anti-Angiopoietin 2 Polyclonal Antibody (PA5-27297), in Mouse Spleen (50 ug of cell lysate) and at a dilution of 1:1000.

product information

Product Type :

Antibody

Product Name :

Angiopoietin 2 Polyclonal Antibody

Catalog # :

PA5-27297

Quantity :

100 µL

Price :

US 470

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.

Immunogen :

Recombinant protein fragment corresponding to a region within amino acids 1 and 496 of Human Angiopoietin 2

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Aliases :

AGPT2; Ang2; Ang-2; angiopoietin 2; angiopoietin-2; angiopoietin-2a; angiopoietin-2B; ANGPT2; endothelial growth factor; Tie2-ligand

more info or order :

Invitrogen product webpage