Vimentin Polyclonal Antibody | Invitrogen PA5-27231 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Vimentin Polyclonal Antibody

catalog :

PA5-27231

quantity :

100 µL

price :

US 554

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:50; fig 1	Fabbri R, Macciocca M, Vicenti R, Paradisi R, Klinger F, Pasquinelli G, et al. Doxorubicin and cisplatin induce apoptosis in ovarian stromal cells obtained from cryopreserved human ovarian tissue. Future Oncol. 2016;12:1699-711 pubmed publisher
	Xu Z, Liu Y, Pan Z, Qin L. Epigenetic upregulation of MNAT1 by SMYD2 is linked to PI3K/AKT activation and tumorigenesis of pancreatic adenocarcinoma. Histol Histopathol. 2024;39:263-277 pubmed publisher
	Sacrist xe1 n G xf3 mez P, Serrano Somavilla A, Castro Espadas L, S xe1 nchez de la Blanca Carrero N, Sampedro N xfa xf1 ez M, Mu xf1 oz De Nova J, et al. Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases. Int J Mol Sci. 2023;24: pubmed publisher
	Xu X, Cai B, Liu Y, Liu R, Li J. MIR503HG silencing promotes endometrial stromal cell progression and metastasis and suppresses apoptosis in adenomyosis by activating the Wnt/β‑catenin pathway via targeting miR‑191. Exp Ther Med. 2023;25:117 pubmed publisher
	Camino T, Lago Baameiro N, Sueiro A, Bravo S, Couto I, Santos F, et al. Brown Adipose Tissue Sheds Extracellular Vesicles That Carry Potential Biomarkers of Metabolic and Thermogenesis Activity Which Are Affected by High Fat Diet Intervention. Int J Mol Sci. 2022;23: pubmed publisher
	Michalik S, Siegerist F, Palankar R, Franzke K, Schindler M, Reder A, et al. Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines. Haematologica. 2022;107:947-957 pubmed publisher
	Wei Z, Hao C, Srinivasagan R, Wu H, Chen J, Fan X. Mitotic Activation Around Wound Edges and Epithelialization Repair in UVB-Induced Capsular Cataracts. Invest Ophthalmol Vis Sci. 2021;62:29 pubmed publisher
	Wang T, Zhang C, Wang S. Ginsenoside Rg3 inhibits osteosarcoma progression by reducing circ_0003074 expression in a miR-516b-5p/KPNA4-dependent manner. J Orthop Surg Res. 2021;16:724 pubmed publisher
	Li X, Dean D, Ferreira A, Nelson S, Hornicek F, Yu S, et al. Establishment and Characterization of a Novel Dedifferentiated Chondrosarcoma Cell Line DDCS2. Cancer Control. 2021;28:10732748211045274 pubmed publisher
	Arafat E, Marzouk R, Mostafa S, Hamed W. Identification of the Molecular Basis of Nanocurcumin-Induced Telocyte Preservation within the Colon of Ulcerative Colitis Rat Model. Mediators Inflamm. 2021;2021:7534601 pubmed publisher
	Barra J, Cerda Infante J, Sandoval L, Gajardo Meneses P, Henriquez J, Labarca M, et al. D-Propranolol Impairs EGFR Trafficking and Destabilizes Mutant p53 Counteracting AKT Signaling and Tumor Malignancy. Cancers (Basel). 2021;13: pubmed publisher
	Batsche E, Yi J, Mauger O, Kornobis E, Hopkins B, Hanmer Lloyd C, et al. CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms. Nucleic Acids Res. 2021;49:6213-6237 pubmed publisher
	Malgundkar S, Burney I, Al Moundhri M, Al Kalbani M, Lakhtakia R, Okamoto A, et al. E2F5 Promotes the Malignancy of Ovarian Cancer Via the Regulation of Hippo and Wnt Pathways. Genet Test Mol Biomarkers. 2021;25:179-186 pubmed publisher
	Jia L, Liu Y, Han Y, Zhou X, Wang F. Differential expression and inhibitory effects of aquaporins on the development of adenomyosis. Mol Med Rep. 2020;22:3840-3850 pubmed publisher
	Malgundkar S, Burney I, Al Moundhri M, Al Kalbani M, Lakhtakia R, Okamoto A, et al. FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer. BMC Cancer. 2020;20:374 pubmed publisher
	Kalisch Smith J, Steane S, Simmons D, Pantaleon M, Anderson S, Akison L, et al. Periconceptional alcohol exposure causes female-specific perturbations to trophoblast differentiation and placental formation in the rat. Development. 2019;146: pubmed publisher
	Krishnamurthy A, Ytterberg A, Sun M, Sakuraba K, Steen J, Joshua V, et al. Citrullination Controls Dendritic Cell Transdifferentiation into Osteoclasts. J Immunol. 2019;202:3143-3150 pubmed publisher
	Guo D, Song X, Guo T, Gu S, Chang X, Su T, et al. Vimentin acetylation is involved in SIRT5-mediated hepatocellular carcinoma migration. Am J Cancer Res. 2018;8:2453-2466 pubmed
	Zhang Y, Zhang Y. Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway. Cell Biol Int. 2018;42:859-866 pubmed publisher
	Kryza T, Silva L, Bock N, Fuhrman Luck R, Stephens C, Gao J, et al. Kallikrein-related peptidase 4 induces cancer-associated fibroblast features in prostate-derived stromal cells. Mol Oncol. 2017;11:1307-1329 pubmed publisher
	Vartholomaiou E, Madon Simon M, Hagmann S, Mühlebach G, Wurst W, Floss T, et al. Cytosolic Hsp90? and its mitochondrial isoform Trap1 are differentially required in a breast cancer model. Oncotarget. 2017;8:17428-17442 pubmed publisher
	Fang S, Yu L, Mei H, Yang J, Gao T, Cheng A, et al. Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail. Oncol Lett. 2016;12:5007-5014 pubmed publisher
	Kolahi K, Louey S, Varlamov O, Thornburg K. Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells. PLoS ONE. 2016;11:e0153522 pubmed publisher

image

image 1 :

Western Blot analysis of Vimentin using anti-Vimentin Polyclonal Antibody (PA5-27231), in 293T Cell (30 ug of cell lysate) and at a dilution of 1:3000.

image 2 :

Confocal immunofluorescence analysis of methanol-fixed HeLa using Vimentin (PA5-27231) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with MA1-25054 (Red) at 1:2000.

image 3 :

Immunohistochemical analysis of paraffin-embedded RT2 xenograft using Vimentin (PA5-27231) antibody at 1:500 dilution.

product information

Product Type :

Antibody

Product Name :

Vimentin Polyclonal Antibody

Catalog # :

PA5-27231

Quantity :

100 µL

Price :

US 554

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:5,000-1:50,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Immunogen :

Recombinant protein fragment corresponding to a region within amino acids 61 and 426 of Human Vimentin

Format :

Liquid

Applications w/Dilutions :

Aliases :

cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus

more info or order :

Invitrogen product webpage