PCNA Polyclonal Antibody | Invitrogen PA5-27214 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PCNA Polyclonal Antibody

catalog :

PA5-27214

quantity :

100 µL

price :

US 496.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

Chinese hamsters, human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 1d	Helle J, Keiler A, Zierau O, Dörfelt P, Vollmer G, Lehmann L, et al. Effects of the aryl hydrocarbon receptor agonist 3-methylcholanthrene on the 17?-estradiol regulated mRNA transcriptome of the rat uterus. J Steroid Biochem Mol Biol. 2017;171:133-143 pubmed publisher
immunocytochemistry; mouse; 1:100; loading ...; fig 2b	Bartova E, Suchankova J, Legartova S, Malyšková B, Hornáček M, Skalníková M, et al. PCNA is recruited to irradiated chromatin in late S-phase and is most pronounced in G2 phase of the cell cycle. Protoplasma. 2017;254:2035-2043 pubmed publisher
	Abramova O, Demyashkin G, Drozhzhina V, Yakovleva N, Kozlovtseva E, Sivovolova T, et al. On the Pathomorphological Pattern of the Efficiency of Photodynamic Therapy of Murine Melanoma B16 Using a New Photosensitizer Based on Chlorin e6 Conjugate with a Prostate-Specific Membrane Antigen. Molecules. 2022;27: pubmed publisher
	Wasbotten R, Dahler A, Mackel J, Morffy Smith C, Rosen D. Murine Respiratory Tract Infection with Classical Klebsiella pneumoniae Induces Bronchus-Associated Lymphoid Tissue. Infect Immun. 2022;90:e0059621 pubmed publisher
	Isaeva E, Beketov E, Demyashkin G, Yakovleva N, Arguchinskaya N, Kisel A, et al. Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules. Int J Mol Sci. 2022;23: pubmed publisher
	Feng C, Wang Q, Deng L, Peng N, Yang M, Wang X. Hsa_circ_0074032 promotes prostate cancer progression through elevating homeobox A1 expression by serving as a microRNA-198 decoy. Andrologia. 2022;54:e14312 pubmed publisher
	He Z, Lei S, Liang F, Tan L, Zhang W, Xie L, et al. Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation. J Oncol. 2021;2021:4475192 pubmed publisher
	Kassa B, Kumar R, Mickael C, Sanders L, Vohwinkel C, Lee M, et al. Endothelial cell PHD2-HIF1α-PFKFB3 contributes to right ventricle vascular adaptation in pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 2021;321:L675-L685 pubmed publisher
	Li S, Jiang T, Cao Q, Liu S. Transmembrane protein ADAM29 facilitates cell proliferation, invasion and migration in clear cell renal cell carcinoma. J Chemother. 2021;33:40-50 pubmed publisher
	An Y, Liu H, Zhou Z, Wang J, Jiang G, Li Z, et al. Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma. Oncol Rep. 2020;44:1596-1604 pubmed publisher
	Mintie C, Musarra A, Singh C, Ndiaye M, Sullivan R, Eickhoff J, et al. Protective Effects of Dietary Grape on UVB-Mediated Cutaneous Damages and Skin Tumorigenesis in SKH-1 Mice. Cancers (Basel). 2020;12: pubmed publisher
	Bersani Amado L, da Rocha B, Schneider L, Ames F, Breithaupt Faloppa A, Araújo G, et al. Nephropathy induced by renal microembolism: a characterization of biochemical and histopathological changes in rats. Int J Clin Exp Pathol. 2019;12:2311-2323 pubmed
	Ragy M, Ahmed S. Protective effects of either C-peptide or l-arginine on pancreatic β-cell function, proliferation, and oxidative stress in streptozotocin-induced diabetic rats. J Cell Physiol. 2019;234:11500-11510 pubmed publisher
	Abe M, Yoshida T, Akiba J, Ikezono Y, Wada F, Masuda A, et al. STAT3 deficiency prevents hepatocarcinogenesis and promotes biliary proliferation in thioacetamide-induced liver injury. World J Gastroenterol. 2017;23:6833-6844 pubmed publisher
	Sozmen M, Devrim A, Kabak Y, Devrim T, Sudagidan M. The Effects of Periostin in a Rat Model of Isoproterenol: Mediated Cardiotoxicity. Cardiovasc Toxicol. 2018;18:142-160 pubmed publisher
	Hasan S, Sultana S. Geraniol attenuates 2-acetylaminofluorene induced oxidative stress, inflammation and apoptosis in the liver of wistar rats. Toxicol Mech Methods. 2015;25:559-73 pubmed publisher
	Gronowicz G, Secor E, Flynn J, Jellison E, Kuhn L. Therapeutic Touch Has Significant Effects on Mouse Breast Cancer Metastasis and Immune Responses but Not Primary Tumor Size. Evid Based Complement Alternat Med. 2015;2015:926565 pubmed publisher

image

image 1 :

Western Blot analysis using anti-PCNA Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG. Primary antibody (PA5-27214) diluted at 1:3000

image 2 :

Confocal immunofluorescence analysis of methanol-fixed HeLa using PCNA (PA5-27214) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with MA1-25054 (Red) at 1:500.

image 3 :

Immunohistochemical analysis of paraffin-embedded KYSE170 xenograft using PCNA (PA5-27214) antibody at 1:500 dilution.

product information

Product Type :

Antibody

Product Name :

PCNA Polyclonal Antibody

Catalog # :

PA5-27214

Quantity :

100 µL

Price :

US 496.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Hamster, Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:500-1:3,000

Species :

Hamster, Human, Mouse, Rat

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

Immunogen :

Recombinant fragment corresponding to a region within amino acids 1 and 253 of Human PCNA

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:500-1:3,000

Aliases :

ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; hypothetical protein LOC515499; MGC8367; Pcna; pcna protein; Pcna/cyclin; PCNAR; POL30; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03; YBR0811; YBR088C

more info or order :

Invitrogen product webpage