RAD51 Polyclonal Antibody | Invitrogen PA5-27195 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

RAD51 Polyclonal Antibody

catalog :

PA5-27195

quantity :

100 µL

price :

US 514

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

fission yeast, human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 4i western blot; mouse; loading ...; fig 2f	Zhang J, Gurusaran M, Fujiwara Y, Zhang K, Echbarthi M, Vorontsov E, et al. The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells. Nat Commun. 2020;11:2055 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig s6c	Liu H, Huang T, Li M, Li M, Zhang C, Jiang J, et al. SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice. Nucleic Acids Res. 2019;47:5670-5683 pubmed publisher
immunocytochemistry; fission yeast; 1:100; loading ...; fig 7d	Manjón E, Edreira T, Muñoz S, Sanchez Y. Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast. Nucleic Acids Res. 2017;45:5269-5284 pubmed publisher
	Ghosh I, Kwon Y, Shabestari A, Chikhale R, Chen J, Wiese C, et al. TLK1-mediated RAD54 phosphorylation spatio-temporally regulates Homologous Recombination Repair. Nucleic Acids Res. 2023;: pubmed publisher
	Zhao S, Huang C, Yang Y, Xu W, Yu Y, Wen C, et al. DNA repair protein FANCD2 has both ubiquitination-dependent and ubiquitination-independent functions during germ cell development. J Biol Chem. 2023;299:102905 pubmed publisher
	Tesfay L, Paul B, Hegde P, Brewer M, Habbani S, JELLISON E, et al. Complementary anti-cancer pathways triggered by inhibition of sideroflexin 4 in ovarian cancer. Sci Rep. 2022;12:19936 pubmed publisher
	Yu R, Hu Y, Zhang S, Li X, Tang M, Yang M, et al. LncRNA CTBP1-DT-encoded microprotein DDUP sustains DNA damage response signalling to trigger dual DNA repair mechanisms. Nucleic Acids Res. 2022;50:8060-8079 pubmed publisher
	Shang Y, Huang J, Li W, Zhang Y, Zhou X, Shao Q, et al. MEIOK21 regulates oocyte quantity and quality via modulating meiotic recombination. FASEB J. 2022;36:e22357 pubmed publisher
	Qin J, Huang T, Wang J, Xu L, Dang Q, Xu X, et al. RAD51 is essential for spermatogenesis and male fertility in mice. Cell Death Discov. 2022;8:118 pubmed publisher
	Moore S, Narayanan D, Simonette R, Bartley B, Doan H, Rady P, et al. Selinexor is a novel inhibitor of DNA damage response in Merkel cell carcinoma. Clin Exp Dermatol. 2022;47:1354-1357 pubmed publisher
	Klomp J, Lee Y, Goodwin C, Papke B, Klomp J, Waters A, et al. CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment. Cell Rep. 2021;37:110060 pubmed publisher
	Liu Y, Yang C, Zhang Z, Jiang H. Gut Microbiota Dysbiosis Accelerates Prostate Cancer Progression Through Increased LPCAT1 Expression and Enhanced DNA Repair Pathways. Front Oncol. 2021;11:679712 pubmed publisher
	Silva V, Saxena J, Nicolini F, Hoare J, Metcalf S, Martin S, et al. Chloroxine overrides DNA damage tolerance to restore platinum sensitivity in high-grade serous ovarian cancer. Cell Death Dis. 2021;12:395 pubmed publisher
	Poveda A, Oaknin A, Romero I, Guerrero Zotano A, Fari xf1 as Madrid L, Rodriguez Freixinos V, et al. A phase I dose-finding, pharmacokinetics and genotyping study of olaparib and lurbinectedin in patients with advanced solid tumors. Sci Rep. 2021;11:4433 pubmed publisher
	Shang Y, Huang T, Liu H, LIU Y, Liang H, Yu X, et al. MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis. Nucleic Acids Res. 2020;48:6624-6639 pubmed publisher
	Huang T, Yuan S, Gao L, Li M, Yu X, Zhang J, et al. The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double-strand break repair. elife. 2020;9: pubmed publisher
	Zhang J, Fujiwara Y, Yamamoto S, Shibuya H. A meiosis-specific BRCA2 binding protein recruits recombinases to DNA double-strand breaks to ensure homologous recombination. Nat Commun. 2019;10:722 pubmed publisher
	Dasgupta Y, Golovine K, Nieborowska Skorska M, Luo L, Matlawska Wasowska K, Mullighan C, et al. Drugging DNA repair to target T-ALL cells. Leuk Lymphoma. 2018;59:1746-1749 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft using Rad51 (PA5-27195) antibody at 1:100 dilution.

image 2 :

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using Rad51 (PA5-27195) antibody at 1:200 dilution.

image 3 :

Western Blot analysis of Rad51 using anti-Rad51 Polyclonal Antibody (PA5-27195), in NIH-3T3 Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.

product information

Product Type :

Antibody

Product Name :

RAD51 Polyclonal Antibody

Catalog # :

PA5-27195

Quantity :

100 µL

Price :

US 514

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat, Zebrafish

Applications :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:500-1:3,000

Species :

Human, Mouse, Rat, Zebrafish

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

Immunogen :

Recombinant fragment corresponding to a region within amino acids 1 and 228 of Human Rad51

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:500-1:3,000

Aliases :

AV304093; BRCA1/BRCA2-containing complex, subunit 5; BRCC5; DNA repair protein RAD51; DNA repair protein RAD51 homolog 1; DNA repair protein RAD51 homolog A; DNA repair protein rhp51; FANCR; homolog to S.cerevisiae; HRAD51; HsRad51; HsT16930; MGC128559 protein; MRMV2; MUT5; rad51; RAD51 homolog; RAD51 homolog (RecA homolog); RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RAD51 recombinase; RAD51 recombinase L homeolog; rad51.1; rad51.L; rad51-1; RAD51A; rad51-a; rad51-b; Reca; RecA family recombinase Rhp51; RecA, E. coli, homolog of; RecA-like protein; recombinase RAD51; recombination protein A; RGD1563603; rhp51; SPAC644.14c; wu:fb38e12; xrad51; XRad51.1; YER095W; zgc:77754

more info or order :

Invitrogen product webpage