ACSL4 Polyclonal Antibody | Invitrogen PA5-27137 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ACSL4 Polyclonal Antibody

catalog :

PA5-27137

quantity :

100 µL

price :

US 514

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; rat; loading ...	Kong Y, Akatsuka S, Motooka Y, Zheng H, Cheng Z, Shiraki Y, et al. BRCA1 haploinsufficiency promotes chromosomal amplification under Fenton reaction-based carcinogenesis through ferroptosis-resistance. Redox Biol. 2022;54:102356 pubmed publisher
western blot; mouse; loading ...; fig s3a	Yang S, Harding A, Sweeney C, Miao D, Swan G, Zhou C, et al. Control of antiviral innate immune response by protein geranylgeranylation. Sci Adv. 2019;5:eaav7999 pubmed publisher
	Du L, Yang H, Ren Y, Ding Y, Xu Y, Zi X, et al. Inhibition of LSD1 induces ferroptosis through the ATF4-xCT pathway and shows enhanced anti-tumor effects with ferroptosis inducers in NSCLC. Cell Death Dis. 2023;14:716 pubmed publisher
	Martino E, Balestrieri A, Aragona F, Bifulco G, Mele L, Campanile G, et al. MiR-148a-3p Promotes Colorectal Cancer Cell Ferroptosis by Targeting SLC7A11. Cancers (Basel). 2023;15: pubmed publisher
	Xie G, Liang Y, Gao W, Wu L, Zhang Y, Ye Z, et al. Artesunate alleviates intracerebral haemorrhage secondary injury by inducing ferroptosis in M1-polarized microglia and suppressing inflammation through AMPK/mTORC1/GPX4 pathway. Basic Clin Pharmacol Toxicol. 2023;132:369-383 pubmed publisher
	Ponnalagu D, Hamilton S, Sanghvi S, Antelo D, Schwieterman N, Hansra I, et al. CLIC4 localizes to mitochondrial-associated membranes and mediates cardioprotection. Sci Adv. 2022;8:eabo1244 pubmed publisher
	LI Y, Fahrmann J, Aftabizadeh M, Zhao Q, Tripathi S, Zhang C, et al. Fatty acid oxidation protects cancer cells from apoptosis by increasing mitochondrial membrane lipids. Cell Rep. 2022;39:110870 pubmed publisher
	Lau A, Rahn J, Chappellaz M, Chung H, Benediktsson H, Bihan D, et al. Dipeptidase-1 governs renal inflammation during ischemia reperfusion injury. Sci Adv. 2022;8:eabm0142 pubmed publisher
	Zheng H, Jiang L, Tsuduki T, Conrad M, Toyokuni S. Embryonal erythropoiesis and aging exploit ferroptosis. Redox Biol. 2021;48:102175 pubmed publisher
	Kuganesan N, Dlamini S, Tillekeratne L, Taylor W. Tumor suppressor p53 promotes ferroptosis in oxidative stress conditions independent of modulation of ferroptosis by p21, CDKs, RB, and E2F. J Biol Chem. 2021;297:101365 pubmed publisher
	Xu P, Chang J, Zhou X, Wang W, Bamkole M, Wong E, et al. GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer's disease. J Exp Med. 2021;218: pubmed publisher
	Noterman M, Chaubey K, Lin Rahardja K, Rajadhyaksha A, Pieper A, Taylor E. Dual-process brain mitochondria isolation preserves function and clarifies protein composition. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
	Abdul Y, Li W, Ward R, Abdelsaid M, Hafez S, Dong G, et al. Deferoxamine Treatment Prevents Post-Stroke Vasoregression and Neurovascular Unit Remodeling Leading to Improved Functional Outcomes in Type 2 Male Diabetic Rats: Role of Endothelial Ferroptosis. Transl Stroke Res. 2020;: pubmed publisher
	Stierwalt H, Ehrlicher S, Robinson M, Newsom S. Diet and Exercise Training Influence Skeletal Muscle Long-Chain acyl-CoA Synthetases. Med Sci Sports Exerc. 2020;52:569-576 pubmed publisher
	Wang W, Hao X, Han L, Yan Z, Shen W, Dong D, et al. Tissue-Specific Ablation of ACSL4 Results in Disturbed Steroidogenesis. Endocrinology. 2019;160:2517-2528 pubmed publisher
	Bach D, Luu T, Kim D, An Y, Park S, Park H, et al. BMP4 Upregulation Is Associated with Acquired Drug Resistance and Fatty Acid Metabolism in EGFR-Mutant Non-Small-Cell Lung Cancer Cells. Mol Ther Nucleic Acids. 2018;12:817-828 pubmed publisher
	Radif Y, Ndiaye H, Kalantzi V, Jacobs R, Hall A, Minogue S, et al. The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells. Mol Cell Biochem. 2018;448:275-286 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft using FACL4 (PA5-27137) antibody at 1:500 dilution.

image 2 :

Confocal immunofluorescence analysis of methanol-fixed HeLa using FACL4 (PA5-27137) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with MA1-25054 (Red) at 1:2000.

image 3 :

Western Blot analysis using anti-FACL4 Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: Hela B: NT2D1. Primary antibody (PA5-27137) diluted at 1:1000

product information

Product Type :

Antibody

Product Name :

ACSL4 Polyclonal Antibody

Catalog # :

PA5-27137

Quantity :

100 µL

Price :

US 514

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:1,000-1:5,000, Western Blot: 1:500-1:10,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants.

Immunogen :

Synthetic peptide corresponding to a region within amino acids 649 and 711 of Human FACL4

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:1,000-1:5,000, Western Blot: 1:500-1:10,000

Aliases :

9430020A05Rik; Acs4; ACSL4; acyl-CoA synthetase 4; acyl-CoA synthetase long-chain family member 4; Arachidonate--CoA ligase; AU018108; Facl4; fatty acid Coenzyme A ligase, long chain 4; fatty acid-Coenzyme A ligase long chain 4; fatty acid-Coenzyme A ligase, long chain 4; fatty-acid-Coenzyme A ligase, long-chain 4; L MRX63; LACS 4; Lacs4; lignoceroyl-CoA synthase; long-chain acyl-CoA synthetase 4; long-chain fatty-acid-Coenzyme A ligase 4; long-chain-fatty-acid--CoA ligase 4; mACS4; MRX63; MRX68

more info or order :

Invitrogen product webpage