Bcl-2 Polyclonal Antibody | Invitrogen PA5-27094 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Bcl-2 Polyclonal Antibody

catalog :

PA5-27094

quantity :

100 µL

price :

US 450.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; rat; 1:250; loading ...; fig 1d	Kattaia A, Abd El Baset S, Mohamed E, Abdul Maksou R, Elfakharany Y. Molecular mechanisms underlying histological and biochemical changes induced by nitrate in rat liver and the efficacy of S-Allylcysteine. Ultrastruct Pathol. 2017;41:10-22 pubmed publisher
	Zohny S, Habib M, Mohamad M, Elayat W, Elhossiny R, El Salam M, et al. Memantine/Aripiprazole Combination Alleviates Cognitive Dysfunction in Valproic Acid Rat Model of Autism: Hippocampal CREB/BDNF Signaling and Glutamate Homeostasis. Neurotherapeutics. 2023;20:464-483 pubmed publisher
	Su Y, Xu J, Chen S, Feng J, Li J, Lei Z, et al. Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway. Transl Androl Urol. 2022;11:1177-1188 pubmed publisher
	Shahin N, Shamma R, Ahmed I. A Nano-Liposomal Formulation of Caffeic Acid Phenethyl Ester Modulates Nrf2 and NF-κβ Signaling and Alleviates Experimentally Induced Acute Pancreatitis in a Rat Model. Antioxidants (Basel). 2022;11: pubmed publisher
	Ye Y, Chang Z, Wang P, Wang Y, Liang J, Chen C, et al. Infarct-preconditioning exosomes of umbilical cord mesenchymal stem cells promoted vascular remodeling and neurological recovery after stroke in rats. Stem Cell Res Ther. 2022;13:378 pubmed publisher
	Long X, Wu H, Zhou Y, Wan Y, Kan X, Gong J, et al. Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats. Front Nutr. 2022;9:852012 pubmed publisher
	Khalil H, Ibrahim H, Ahmed E, Emeka P, Alhaider I. Orientin, a Bio-Flavonoid from Trigonella hamosa L., Regulates COX-2/PGE-2 in A549 Cell Lines via miR-26b and miR-146a. Pharmaceuticals (Basel). 2022;15: pubmed publisher
	Ashour H, Hashem H, Khowailed A, Rashed L, Hassan R, Soliman A. Necrostatin-1 mitigates renal ischaemia-reperfusion injury - time dependent - via aborting the interacting protein kinase (RIPK-1)-induced inflammatory immune response. Clin Exp Pharmacol Physiol. 2022;49:501-514 pubmed publisher
	Hu X, Lou T, Yuan C, Wang Y, Tu X, Wang Y, et al. Effects of lncRNA ANRIL-knockdown on the proliferation, apoptosis and cell cycle of gastric cancer cells. Oncol Lett. 2021;22:621 pubmed publisher
	Alzahrani A, Rajendran P, Veeraraghavan V, Hanieh H. Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways. Int J Mol Sci. 2021;22: pubmed publisher
	Elblehi S, El Sayed Y, Soliman M, Shukry M. Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways. Animals (Basel). 2021;11: pubmed publisher
	Fakhrinnisa T, Susilo I, Mustika A, Sofyan M. Effect of Intravenous Glutamine on Caspase-12 Expression in the Apoptosis of the Glomerular Epithelial Cells of Male Rats Exposed to Cisplatin. Asian Pac J Cancer Prev. 2021;22:457-462 pubmed publisher
	Rajendran P, Ammar R, Al Saeedi F, Mohamed M, ElNaggar M, Al ramadan S, et al. Kaempferol Inhibits Zearalenone-Induced Oxidative Stress and Apoptosis via the PI3K/Akt-Mediated Nrf2 Signaling Pathway: In Vitro and In Vivo Studies. Int J Mol Sci. 2020;22: pubmed publisher
	Xu Q, Yan P, Duan X, Wu X, Chen X, Luo M, et al. Human umbilical cord-derived mesenchymal stem cells and human cord blood mononuclear cells protect against cisplatin-induced acute kidney injury in rat models. Exp Ther Med. 2020;20:145 pubmed publisher
	Kinarivala N, Morsy A, Patel R, Carmona A, Sajib M, Raut S, et al. An iPSC-Derived Neuron Model of CLN3 Disease Facilitates Small Molecule Phenotypic Screening. ACS Pharmacol Transl Sci. 2020;3:931-947 pubmed publisher
	Li X, Liao J, Su X, Li W, Bi Z, Wang J, et al. Human urine-derived stem cells protect against renal ischemia/reperfusion injury in a rat model via exosomal miR-146a-5p which targets IRAK1. Theranostics. 2020;10:9561-9578 pubmed publisher
	Konac E, Varol N, Kiliccioglu I, Bilen C. Synergistic effects of cisplatin and proteasome inhibitor bortezomib on human bladder cancer cells. Oncol Lett. 2015;10:560-564 pubmed

image

image 1 :

Immunohistochemical analysis of paraffin-embedded Breast ca using BCL2 (PA5-27094) antibody at 1:250 dilution.

image 2 :

Western Blot analysis of BCL2 using anti-BCL2 Polyclonal Antibody (PA5-27094), in Jurkat Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.

product information

Product Type :

Antibody

Product Name :

Bcl-2 Polyclonal Antibody

Catalog # :

PA5-27094

Quantity :

100 µL

Price :

US 450.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat, Zebrafish

Applications :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Species :

Human, Mouse, Rat, Zebrafish

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Immunogen :

Synthetic peptide corresponding to a region within amino acids 1 and 48 of Human BCL2

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Aliases :

apoptosis regulator Bcl-2; AW986256; B cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; BCL2; bcl-2; BCL2 apoptosis regulator; Bcl-2 protein; BCL2, apoptosis regulator; Bcl2-like protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; OTTHUMP00000163680; PPP1R50; protein phosphatase 1, regulatory subunit 50

more info or order :

Invitrogen product webpage