TFAM Polyclonal Antibody | Invitrogen PA5-23776 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TFAM Polyclonal Antibody

catalog :

PA5-23776

quantity :

400 uL

price :

US 464.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 4

Reference
Han M, Frizzi K, Ellis R, Calcutt N, FIELDS J. Prevention of HIV-1 TAT Protein-Induced Peripheral Neuropathy and Mitochondrial Disruption by the Antimuscarinic Pirenzepine. Front Neurol. 2021;12:663373 pubmed publisher
FIELDS J, Swinton M, Carson A, Soontornniyomkij B, Lindsay C, Han M, et al. Tenofovir disoproxil fumarate induces peripheral neuropathy and alters inflammation and mitochondrial biogenesis in the brains of mice. Sci Rep. 2019;9:17158 pubmed publisher
Swinton M, Carson A, Telese F, Sanchez A, Soontornniyomkij B, Rad L, et al. Mitochondrial biogenesis is altered in HIV+ brains exposed to ART: Implications for therapeutic targeting of astroglia. Neurobiol Dis. 2019;130:104502 pubmed publisher
Jevtic V, Kindle P, Avilov S. SYBR Gold dye enables preferential labelling of mitochondrial nucleoids and their time-lapse imaging by structured illumination microscopy. PLoS ONE. 2018;13:e0203956 pubmed publisher

product information

Product Type :

Antibody

Product Name :

TFAM Polyclonal Antibody

Catalog # :

PA5-23776

Quantity :

400 uL

Price :

US 464.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography, Protein A

Host :

Rabbit

Reactivity :

Human

Applications :

Flow Cytometry: 1:10-1:50, Immunocytochemistry: 1:10-1:50, Immunohistochemistry (Paraffin): 1:50-1:100, Western Blot: 1:1,000

Species :

Human

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Mitochondrial transcription factor A (TFAM) is a crucial protein encoded by nuclear genes, but it is transported from the cytoplasm to mitochondria where it plays a key role in the maintenance and expression of mitochondrial DNA (mtDNA). It regulates the replication and transcription of mtDNA, ensuring the proper functioning of mitochondrial processes. Structurally, TFAM contains a molecular region known as the LC3 interacting region (LIR) motif, which allows it to bind to autophagy proteins such as LC3. This interaction is essential for the autolysosomal pathway, helping to eliminate leaked mtDNA and limit inflammation. TFAM levels vary across tissues, with notable effects on mitochondrial function; high levels can repress mtDNA expression in certain tissues, such as skeletal muscle, and contribute to deficiencies in oxidative phosphorylation (OXPHOS). Conversely, in other tissues like the heart, increased mtDNA copy number maintains a balanced TFAM-to-mtDNA ratio, preserving OXPHOS capacity. TFAM is integral to the packaging, stability, and replication of the mitochondrial genome, and disruptions in TFAM can have severe effects, including implications in neurodegeneration and other diseases related to mtDNA stress.

Immunogen :

KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human TFAM

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:10-1:50, Immunocytochemistry: 1:10-1:50, Immunohistochemistry (Paraffin): 1:50-1:100, Western Blot: 1:1,000

Aliases :

AI661103; Hmgts; Mitochondrial transcription factor 1; mitochondrial transcription factor A; MTTF1; mtTFA; TCF6; TCF-6; TCF6L1; TCF6L2; TCF6L3; Testis-specific high mobility group protein; testis-specific HMG-box protein m-tsHMG; TFAM; Transcription factor 6; transcription factor 6-like 1; transcription factor 6-like 2; transcription factor 6-like 2 (mitochondrial transcription factor); transcription factor 6-like 3; transcription factor A, mitochondrial; tsHMG; TS-HMG