AMPK alpha-2 Polyclonal Antibody | Invitrogen PA5-21494 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

AMPK alpha-2 Polyclonal Antibody

catalog :

PA5-21494

quantity :

100 µL

price :

US 514

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 1d, 1e, 1f	Dufeys C, Daskalopoulos E, Castanares Zapatero D, Conway S, Ginion A, Bouzin C, et al. AMPKα1 deletion in myofibroblasts exacerbates post-myocardial infarction fibrosis by a connexin 43 mechanism. Basic Res Cardiol. 2021;116:10 pubmed publisher
	André E, De Pauw A, Verdoy R, Brusa D, Bouzin C, Timmermans A, et al. Changes of Metabolic Phenotype of Cardiac Progenitor Cells During Differentiation: Neutral Effect of Stimulation of AMP-Activated Protein Kinase. Stem Cells Dev. 2019;28:1498-1513 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue using PA5-21494 anti-AMPK alpha 2 antibody at 1:500 dilution.

image 2 :

Immunofluorescence analysis of paraformaldehyde-fixed A431 cells using PA5-21494 anti-AMPK alpha 2 antibody at 1:200 dilution.

product information

Product Type :

Antibody

Product Name :

AMPK alpha-2 Polyclonal Antibody

Catalog # :

PA5-21494

Quantity :

100 µL

Price :

US 514

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:500-1:3,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Immunogen :

Recombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2 (Uniprot ID#P54646)

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:500-1:3,000

Aliases :

2310008I11Rik; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; A830082D05; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP-activated protein kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMPK; AMPK alpha 2; AMPK alpha-2 chain; AMPK subunit alpha-2; AMPK2; AMPKa2; AMPKalpha2; AMPK-alpha-2 chain; EC 2.7.11.1; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; PRKAA; Prkaa2; protein kinase AMP-activated catalytic subunit alpha 2; protein kinase, AMP-activated, alpha 2 catalytic subunit

more info or order :

Invitrogen product webpage