PUF60 Polyclonal Antibody | Invitrogen PA5-21411 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PUF60 Polyclonal Antibody

catalog :

PA5-21411

quantity :

100 µL

price :

US 522

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 7s1b western blot; mouse; loading ...; fig 7s1b	Kew C, Huang W, Fischer J, Ganesan R, Robinson N, Antebi A. Evolutionarily conserved regulation of immunity by the splicing factor RNP-6/PUF60. elife. 2020;9: pubmed publisher

image

image 1 :

Immunofluorescence analysis of paraformaldehyde-fixed A431 cells using PA5-21411 anti-PUF60 antibody at 1:500 dilution.

image 2 :

Immunohistochemical analysis of paraffin-embedded oral tissue tissue using PA5-21411 anti-PUF60 antibody at 1:100 dilution.

product information

Product Type :

Antibody

Product Name :

PUF60 Polyclonal Antibody

Catalog # :

PA5-21411

Quantity :

100 µL

Price :

US 522

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Species :

Human, Mouse

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

PUF60 encodes a protein that is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.

Immunogen :

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 346 and 523 (Q9UHX1) of PUF60

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Aliases :

2410104I19Rik; 2810454F19Rik; 60 kDa poly(U)-binding-splicing factor; FBP interacting repressor; FBP-interacting repressor; FIR; FLJ31379; FUSE-binding protein-interacting repressor; LOW QUALITY PROTEIN: poly(U)-binding-splicing factor PUF60; poly(U) binding splicing factor 60; poly(U) binding splicing factor 60KDa; poly(U)-binding-splicing factor PUF60; poly-U binding splicing factor 60; poly-U binding splicing factor 60K; poly-U binding splicing factor 60KDa; PUF60; pyrimidine tract binding splicing factor; RNA-binding protein Siah-BP; Ro ribonucleoprotein-binding protein 1; Ro-binding protein 1; RoBP1; ROBPI; Siah binding protein 1; siah binding protein 1; FBP interacting repressor; pyrimidine tract binding splicing factor; Ro ribonucleoprotein-binding protein 1; siah-binding protein 1; SIAHBP1; Siah-BP1; similar to fuse-binding protein-interacting repressor isoform a; VRJS

more info or order :

Invitrogen product webpage