GAD67 Polyclonal Antibody | Invitrogen PA5-21397 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

GAD67 Polyclonal Antibody

catalog :

PA5-21397

quantity :

100 µL

price :

US 470

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...	Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, et al. ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity. Transl Psychiatry. 2021;11:361 pubmed publisher
western blot; human; 1:2000; fig 4	Urrutia M, Fernandez S, Gonzalez M, Vilches R, Rojas P, Vásquez M, et al. Overexpression of Glutamate Decarboxylase in Mesenchymal Stem Cells Enhances Their Immunosuppressive Properties and Increases GABA and Nitric Oxide Levels. PLoS ONE. 2016;11:e0163735 pubmed publisher
	Lyu J, Nagarajan R, Kambali M, Wang M, Rudolph U. Selective inhibition of somatostatin-positive dentate hilar interneurons induces age-related cellular changes and cognitive dysfunction. PNAS Nexus. 2023;2:pgad134 pubmed publisher
	Vollbrecht P, Nesbitt K, Addis V, Boulnemour K, Micheli D, Smith K, et al. Differential regulation of nucleus accumbens glutamate and GABA in obesity-prone and obesity-resistant rats. J Neurochem. 2023;164:499-511 pubmed publisher
	Pai V, Cooper B, Levin M. Screening Biophysical Sensors and Neurite Outgrowth Actuators in Human Induced-Pluripotent-Stem-Cell-Derived Neurons. Cells. 2022;11: pubmed publisher
	Wu J, Yeung J, Rahimi Balaei M, Wu S, Zoghbi H, Goldowitz D. The Transcription Factor Pou3f1 Sheds Light on the Development and Molecular Diversity of Glutamatergic Cerebellar Nuclear Neurons in the Mouse. Front Mol Neurosci. 2022;15:921901 pubmed publisher
	Boczek T, Lisek M, Ferenc B, Wiktorska M, Ivchevska I, Zylinska L. Region-specific effects of repeated ketamine administration on the presynaptic GABAergic neurochemistry in rat brain. Neurochem Int. 2015;91:13-25 pubmed publisher

image

image 1 :

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using PA5-21397 anti-GAD67 antibody at 1:200 dilution.

product information

Product Type :

Antibody

Product Name :

GAD67 Polyclonal Antibody

Catalog # :

PA5-21397

Quantity :

100 µL

Price :

US 470

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

Immunogen :

Recombinant fragment corresponding to a region within amino acids 5 and 216 of GAD67 (Uniprot ID#Q99259)

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:500-1:3,000

Aliases :

67 kDa glutamic acid decarboxylase; 67kDa glutamate decarboxylase 1; CPSQ1; EGK_04525; EP10; FLJ45882; GAD; GAD 1; GAD 67; GAD1; Gad-1; GAD25; GAD44; Gad67; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 isoform GAD67; glutamate decarboxylase 1 variant GAD67NT; glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); glutamic acid decarboxylase; glutamic acid decarboxylase 1; glutamic acid decarboxylase isoform 67; OTTHUMP00000041055; SCP

more info or order :

Invitrogen product webpage