Nephrin Polyclonal Antibody | Invitrogen PA5-20330 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Nephrin Polyclonal Antibody

catalog :

PA5-20330

quantity :

100 ug

price :

US 474.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:2000; loading ...; fig 3a	Lin C, Hsu Y, Huang Y, Shih Y, Wang C, Chiang W, et al. A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction. EMBO Mol Med. 2019;11: pubmed publisher
	Gao W, Liu Y, Fan L, Zheng B, Jefferson J, Wang S, et al. Role of γ-adducin in actin cytoskeleton rearrangements in podocyte pathophysiology. Am J Physiol Renal Physiol. 2021;320:F97-F113 pubmed publisher
	Petrosyan A, Cravedi P, Villani V, Angeletti A, Manrique J, Renieri A, et al. A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier. Nat Commun. 2019;10:3656 pubmed publisher
	Suzuki A, Minamide R, Iwata J. WNT/β-catenin signaling plays a crucial role in myoblast fusion through regulation of nephrin expression during development. Development. 2018;145: pubmed publisher
	Haley K, Kronenberg N, Liehm P, Elshani M, Bell C, Harrison D, et al. Podocyte injury elicits loss and recovery of cellular forces. Sci Adv. 2018;4:eaap8030 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Nephrin Polyclonal Antibody

Catalog # :

PA5-20330

Quantity :

100 ug

Price :

US 474.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunohistochemistry (Paraffin): 1-5 ug/mL, Immunohistochemistry (PFA fixed): 10-20 ug/mL, Western Blot: 1 ug/mL

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

4 C

Description :

Primary steroid resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss. Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2. Nephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Recent studies have suggested that mutations in the gene for Nephrin reportedly lead to congenital nephrosis. Three novel podocyte proteins, Podocin, Nephrin and alpha Actinin 4 have been identified in congenital and experimental models of proteinuria. The role of Nephrin in anti apoptotic activity in podocyte slit diaphragm is believed to be associated with vascular endothelial derived growth factors VEGF signaling. Nephrin seems to play a role in the development or function of the kidney glomerular filtration barrier and it may anchor the podocyte slit diaphragm to the actin cytoskeleton.

Immunogen :

A 14 amino acid peptide from near the carboxy terminus of human Nephrin.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1-5 ug/mL, Immunohistochemistry (PFA fixed): 10-20 ug/mL, Western Blot: 1 ug/mL

Aliases :

CNF; Nephrin; nephrin 1; NephrinB; nephrosis 1 homolog, nephrin; nephrosis 1, congenital, Finnish type (nephrin); nephrosis 1, nephrin; NPHN; NPHS1; NPHS1 nephrin; NPHS1, nephrin; renal glomerulus-specific cell adhesion receptor

more info or order :

Invitrogen product webpage