N-cadherin Polyclonal Antibody | Invitrogen PA5-19486 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

N-cadherin Polyclonal Antibody

catalog :

PA5-19486

quantity :

100 µg

price :

US 506.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:300 immunocytochemistry; human; 1:200; loading ...; fig 1c western blot; human; 1:1000; loading ...; fig 1e	Fang S, Yu L, Mei H, Yang J, Gao T, Cheng A, et al. Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail. Oncol Lett. 2016;12:5007-5014 pubmed publisher
	Xu X, Zhang X, Gao L, Liu C, You K. Neonatal Hyperoxia Downregulates Claudin-4, Occludin, and ZO-1 Expression in Rat Kidney Accompanied by Impaired Proximal Tubular Development. Oxid Med Cell Longev. 2020;2020:2641461 pubmed publisher
	Bacci L, Aiello A, Ripoli C, Loria R, Pugliese D, Pierconti F, et al. H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer. Int J Mol Sci. 2019;20: pubmed publisher
	Abdala Valencia H, Kountz T, Marchese M, Cook Mills J. VCAM-1 induces signals that stimulate ZO-1 serine phosphorylation and reduces ZO-1 localization at lung endothelial cell junctions. J Leukoc Biol. 2018;104:215-228 pubmed publisher
	Chen Y, Lu X, Montoya Durango D, Liu Y, Dean K, Darling D, et al. ZEB1 Regulates Multiple Oncogenic Components Involved in Uveal Melanoma Progression. Sci Rep. 2017;7:45 pubmed publisher

image

image 1 :

Western blot analysis of Mouse Brain Tissue Lysate using PA5-19486, N Cadherin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19486 staining of Rat Brain Tissue Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

image 2 :

Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Liver Cancer tissue using PA5-19486, anti-N Cadherin antibody. Primary antibody was used at a concentration of 1 ug/ml and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6 / 20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.

product information

Product Type :

Antibody

Product Name :

N-cadherin Polyclonal Antibody

Catalog # :

PA5-19486

Quantity :

100 µg

Price :

US 506.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 1 µg/mL, Immunoprecipitation: Assay-Dependent, Western Blot: 1 µg/mL

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

-20°C or -80°C if preferred

Description :

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

Immunogen :

Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human N Cadherin.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 1 µg/mL, Immunoprecipitation: Assay-Dependent, Western Blot: 1 µg/mL

Aliases :

bib; cadherin 2; cadherin 2 L homeolog; cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, neuronal; cadherin 2, type 1 preproprotein; cadherin 2, type 1, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal) L homeolog; cadherin 2, type 1, N-cadherin a (neuronal); Cadherin2; cadherin-2; Cadherin-2A; Cadherin-N; calcium-dependent adhesion protein, neuronal; CD325; CDH2; cdh2.L; cdh2a; cdh2-a; cdh2-b; CDHN; cdhn-a; CDw325; CDw325 antigen; cell adhesion molecule; glass onion; glo; I79_014914; labyrinth; lyr; NCAD; N-Cad; N-cadherin; N-cadherin 1; N-cadherin A; N-cadherin precursor; N-cadherin-2; neural cadherin; Neural cadherin A; neural cadherin-2; neural calcium-dependent cell adhesion molecule; neural-cadherin; pac; Parachute; unnamed protein product; wu:fb47h04; XELAEV_18031953mg; ZNCAD

more info or order :

Invitrogen product webpage